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目的建立HPLC测定芍甘胶囊中甘草酸和甘草苷含量的方法。方法色谱柱为Kromasil C18(250 mm×4.6 mm,5μm)。甘草酸单铵盐以甲醇-0.2 mol.l-1醋酸铵溶液-冰醋酸(65:35:1)为流动相,检测波长250 nm,流速1.0 ml.min-1;甘草苷以乙腈-0.5%冰醋酸(18:82)为流动相,检测波长276 nm,流速1.0 ml.min-1。结果甘草酸单铵盐和甘草苷的线性范围分别为0.52~2.60、0.30~1.50μg(r=0.9998);平均回收率分别为98.1%(RSD=1.32%,n=5)、97.0%(RSD=1.67%,n=5)。结论所建方法灵敏、准确、专属性强,可作为芍甘胶囊的质量控制方法。
Objective To establish a HPLC method for determination of glycyrrhizic acid and glycyrrhizin in Fugan capsules. Method The column was Kromasil C18 (250 mm×4.6 mm, 5 μm). The monoammonium glycyrrhizinate was methanol-0.2 mol.l-1 ammonium acetate solution-glacial acetic acid (65:35:1) as the mobile phase, the detection wavelength was 250 nm, the flow rate was 1.0 ml.min-1, and the glycyrrhizin was acetonitrile-0.5. % glacial acetic acid (18:82) as mobile phase, detection wavelength 276 nm, flow rate 1.0 ml.min-1. Results The linear ranges of monoammonium glycyrrhizinate and glycyrrhizin were 0.52 to 2.60 and 0.30 to 1.50 μg, respectively (r=0.9998). The average recoveries were 98.1% (RSD=1.32%, n=5) and 97.0% (RSD, respectively). =1.67%, n=5). Conclusion The method is sensitive, accurate, and specific. It can be used as a quality control method for Fugan capsule.