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本文在室内研究了分离自小猿叶甲的一株球孢白僵菌(SCAU-BB01D)在不同温度和湿度条件下对小猿叶甲成虫和2龄幼虫的致病力。结果显示温度和相对湿度对白僵菌的致病力有显著影响。在17℃和29℃,小猿叶甲成虫和2龄幼虫的死亡率显著低于其他温度;在23℃下,成虫接菌后第14天的累计死亡率达到最高为95.60%;在23℃和26℃下,2龄幼虫接菌后第10天达到最高累计死亡率96%。随着相对湿度的提高,球孢白僵菌对小猿叶甲的致病力显著增加,当相对湿度从50%增加至100%时,成虫第14天的累计死亡率从55.70%增加到88.80%,2龄幼虫第10天的累计死亡率从62.00%增加到96.00%。机率值分析结果表明,在17、20、23、26和29℃温度下,白僵菌对小猿叶甲成虫和2龄幼虫的LT50分别为13.63、10.27、8.05、8.87、12.41天和6.06、5.72、4.90、4.86、6.73天。随着相对湿度的升高,球孢白僵菌对小猿叶甲的致死中时(LT50)呈缩短的趋势,当相对湿度为50%、60%、70%、80%、90%、100%时,成虫和2龄幼虫的LT50分别为12.97、10.97、10.13、9.79、9.14、8.29天和7.68、6.92、5.76、5.57、5.48、4.82天。由实验结果可以得知,23~26℃是球孢白僵菌感染小猿叶甲最适宜的温度范围,环境湿度越高越有利于病原菌侵染,当相对湿度大于90%时,最有利于球孢白僵菌对小猿叶甲的感染。
In this paper, the virulence of a strain of Beauveria bassiana (SCAU-BB01D) isolated from Pedicularis aurantiacus to the adults and 2nd instar larvae of P. chinensis under different temperature and humidity conditions was studied indoors. The results showed that temperature and relative humidity had significant effects on the virulence of Beauveria bassiana. At 17 ℃ and 29 ℃, the mortality of P. marina adults and 2nd instar larvae was significantly lower than that of other temperatures. The highest mortality rate reached 95.60% on the 14th day after inoculation at 23 ℃. At 23 ℃ And 26 ℃, the 2nd instar larvae reached the highest cumulative mortality of 96% on the 10th day after inoculation. With the increase of relative humidity, the pathogenicity of Beauveria bassiana increased significantly from 50% to 50%, and the cumulative mortality increased from 55.70% to 88.80% when the relative humidity increased from 50% to 100% %, The cumulative mortality of the 2nd instar larvae on the 10th day increased from 62.00% to 96.00%. The results of probability value analysis showed that the LT50 of Beauveria bassiana was 13.63, 10.27, 8.05, 8.87, 12.41 and 6.06 respectively for the leafhopper adults and the second instar larvae at 17, 20, 23, 26 and 29 ℃, 5.72, 4.90, 4.86, 6.73 days. With the increase of relative humidity, Beauveria bassiana tended to shorten the LT50 when relative humidity was 50%, 60%, 70%, 80%, 90%, 100 %, The LT50 of adult and 2nd instar larvae were 12.97,10.97,10.13,9.79,9.14,8.29 days and 7.68,6.92,5.76,5.57,5.48,4.82 days, respectively. From the experimental results, it can be learned that 23 ~ 26 ℃ is the most suitable temperature range for Beauveria bassiana infected with Beauveria bassiana. The higher the ambient humidity, the more conducive to pathogen infection. When the relative humidity is more than 90%, the most favorable Infection of Beauveria bassiana to Pedigree.