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通过比较茄青枯拉尔氏菌和柑橘黄单胞菌柑橘致病变种菌量、噻唑蓝和刃天青的浓度、抑菌剂与菌体作用时间等因素对吸光值的影响,优化噻唑蓝和刃天青法检测抑菌剂抑菌活性的条件,即:细菌初始浓度为105～106CFU/mL,噻唑蓝和刃天青浓度分别为0.5mg/mL和0.02mg/mL,抑菌剂作用时间16～24 h。运用优化的噻唑蓝和刃天青法检测了植物提取物和分离物对2种细菌的抑菌活性,2种方法得到的结果一致。以茄青枯拉尔氏菌为靶标菌,对于无色化合物EE-1和土黄色化合物EE-2,2种方法测定的最低抑菌浓度相同,分别为10μg/mL和20μg/mL。表明噻唑蓝和刃天青法能快速、准确地测定微量植物成分的抑菌活性。
By comparing the effects of citrus pathogenic fungi, the concentration of thiazole blue and resazurin, the antibacterial agent and the time of action of bacteriostasis on the absorbance of Citrus solani and Citrus caused by Xanthomonas oryzae, The antibacterial activity of bacteriostasis was tested by using resazurin and resazurin method. The initial bacteriocidal concentration was 105-106CFU / mL, the concentrations of thiazolyl azole and resazurin were 0.5mg / mL and 0.02mg / mL respectively, Time 16 ~ 24 h. The antibacterial activities of plant extracts and isolates on two kinds of bacteria were tested by the optimized thiazolyl blue and resazurin method. The results obtained by the two methods were consistent. The lowest inhibitory concentrations (MICs) were 10 μg / mL and 20 μg / mL, respectively, for the B. thaliana isolates as EE-1 and EE-2. It is indicated that thiazole blue and resazurin can rapidly and accurately determine the antibacterial activities of trace elements.