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目的:研究甲硝唑、替硝唑在授乳妇女乳汁中的分泌。方法:高效液相色谱法。Ultraspbere-ODS柱,甲硝唑乳药分析用流动相为乙腈-0.1％冰醋酸(50:50),替硝唑乳药分析用流动相为乙腈-水(60:40),流速1.0mL·min~(-1) ,检测波长318nm。结果:甲硝唑乳汁药物浓度在2h时为(10.11±5.390)μg·mL~(-1),8h时尚可测得(4.478±2.669)μg·mL~(-1)。替硝唑乳汁药物浓度在2h时为(14.17±5.746)μg·mL~(-1),8h时尚可测得(9.364±5.123)μg·mL~(-1)。结论:本文建立的高效液相色谱法简单、灵敏,适用于乳药浓度的测定,建议授乳妇女避开给药时间哺乳。
Objective: To study the secretion of metronidazole and tinidazole in breast milk of lactating women. Methods: High performance liquid chromatography. Ultraspbere-ODS column, the mobile phase of metronidazole emulsion was acetonitrile-0.1% glacial acetic acid (50:50), tinidazole emulsion was acetonitrile-water (60:40) min ~ (-1), detection wavelength 318nm. Results: The concentration of metronidazole in milk was (10.11 ± 5.390) μg · mL -1 at 2h, and it was 4.478 ± 2.669 μg · mL -1 at 8h. The concentration of tinidazole in milk was (14.17 ± 5.746) μg · mL -1 at 2h, and 9.364 ± 5.123 μg · mL -1 at 8h. Conclusion: The high performance liquid chromatography established in this paper is simple and sensitive, suitable for the determination of the concentration of milk, lactating women are advised to avoid breast-feeding time.