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目的:探讨胃癌组织HIF-1α的表达与幽门螺杆菌(Hp)感染的相关性,以及两者与胃癌临床病理特征之间的关系。方法:采用快速尿素酶试验和组织学改良Giemsa染色法联合检测130例胃癌组织与70例慢性胃炎组织中Hp感染情况,采用免疫组织化学PV9000法,检测130例胃癌组织与70例慢性胃炎组织中HIF-1α蛋白的阳性表达情况。结果:胃癌组明确感染Hp的患者有100例,Hp阳性感染率为72.0%,明显高于胃炎组的46.0%,χ2=9.703,P<0.01;胃癌组HIF-1α蛋白阳性表达率为56.0%,明显高于胃炎组的14.0%,χ2=24.138,P<0.01;Hp(+)组HIF-1α蛋白的阳性表达率为63.9%,明显高于Hp(-)组的35.7%,χ2=6.495,P<0.05;胃癌组织中Hp感染与HIF-1α蛋白表达之间存在正相关,r=0.255,P<0.05;Hp感染与胃癌组织浸润深度及有无淋巴结转移有关,P值均<0.05,而与组织分化程度无关,P>0.05。HIF-1α蛋白的表达水平与胃癌组织浸润深度(P<0.05)及有无淋巴结转移(P<0.01)有关,而与组织分化程度无关,P>0.05。结论:Hp感染与HIF-1α蛋白表达可能在胃癌的发生中起协同作用,并与胃癌的浸润深度及淋巴结转移等密切相关。
Objective: To investigate the relationship between the expression of HIF-1α and the infection of Helicobacter pylori (Hp) in gastric cancer and the relationship between them and the clinicopathological features of gastric cancer. Methods: Hp infection in 130 gastric cancer tissues and 70 chronic gastritis tissues was detected by rapid urease test and histological modified Giemsa staining. The immunohistochemical PV9000 method was used to detect the expression of Hp in 130 gastric cancer tissues and 70 chronic gastritis tissues HIF-1α protein expression of the positive. Results: There were 100 cases of Hp infection in gastric cancer group, the positive rate of Hp infection was 72.0%, significantly higher than 46.0% in gastritis group (χ2 = 9.703, P <0.01). The positive rate of HIF-1α protein in gastric cancer group was 56.0% , Which was significantly higher than that in gastritis group (14.0%, χ2 = 24.138, P <0.01). The positive expression rate of HIF-1α protein in Hp (+) group was 63.9%, significantly higher than 35.7% in Hp , P <0.05; There was a positive correlation between Hp infection and HIF-1αprotein expression in gastric cancer tissues, r = 0.255, P <0.05.Hp infection was associated with the depth of invasion and lymph node metastasis, But not with the degree of differentiation, P> 0.05. The expression of HIF-1α protein was correlated with the depth of invasion (P <0.05) and lymph node metastasis (P <0.01), but not with the degree of differentiation (P> 0.05). Conclusions: Hp infection and HIF-1α protein expression may play a synergistic role in the pathogenesis of gastric cancer, which is closely related to the depth of invasion and lymph node metastasis.