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目的观察应用安宫牛黄丸治疗脑外伤后出现血脑屏障的损伤与脑水肿的效果。方法参照Feeney法来建立闭合脑损伤的大鼠模型,围绕其脑外伤性的脑水肿时出现血脑屏障受损的关键环节,探讨脑含水量与BBB的通透性,包括神经细胞的突触数量所受的影响,从而阐释开窍醒脑机制。结果同假手术组进行对比,模型组和尼莫通组在损伤侧脑的含水量与脑皮质的EB含量均显著增高(P<0.01);同模型组和尼莫通组进行对比,安宫牛黄组在损伤侧脑的含水量与脑皮质的EB含量均显著下降(P<0.05);同假手术组进行对比,模型组和尼莫通组在损伤侧脑的皮质电镜下,其突触密度呈显著下降(P<0.01);同模型组和尼莫通组进行对比,安宫牛黄组在损伤侧脑的皮质电镜下,其突触密度呈显著增高(P<0.05)。结论应用安宫牛黄丸进行治疗,能够减轻脑水肿,并保护其血脑屏障,同时降低其毛细血管的通透性,从而提高其脑组织对于缺血以及缺氧等的耐受性,达到保护其脑组织的目的 。
Objective To observe the effect of applying Angong Niuhuang Wan on blood-brain barrier injury and cerebral edema after traumatic brain injury. Methods According to the method of Feeney, a rat model of brain injury with closed brain was established. The key part of the damage of the BBB around traumatic brain edema was discussed. The brain water content and BBB permeability, including synapse The impact of the number, thus explaining the mechanism of resuscitation and refreshing. Results Compared with the sham-operated group, the water content in brain and the EB content of cerebral cortex in model group and nimotop group were significantly increased (P <0.01), compared with model group and nimotop group, Compared with the sham-operation group, the water content in brain and the content of EB in cerebral cortex of the bezoar group were significantly decreased (P <0.05). In the model group and the nimotop group, the synapses (P <0.01). Compared with the model group and the nimotop group, the synaptic density in Angong Niuhuang group was significantly increased (P <0.05) by cortical electron microscopy. Conclusions The application of Angong Niuhuang Wan for treatment can reduce brain edema, protect the blood-brain barrier and reduce the permeability of capillaries, thereby increasing the tolerance of brain tissue to ischemia and hypoxia and thus protecting The purpose of their brain tissue.