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目的研究氟致人肝细胞DNA损伤与细胞凋亡之间的关系。方法采用40、80、160μg/ml氟化钠对培养的人肝细胞进行染毒,12h后用单细胞凝胶电泳技术(SCGE)和流式细胞术(FCM)检测氟化物对人肝细胞DNA损伤和细胞凋亡情况。结果各氟染毒组人肝细胞DNA损伤程度明显高于对照组,并且随着染氟剂量的增高而增大;中、高剂量氟组人肝细胞凋亡百分率均明显高于对照组。同时,高剂量氟组与低剂量氟组之间细胞DNA损伤程度和凋亡率的差异亦有统计学意义。结论氟可导致人肝细胞DNA损伤,诱导细胞凋亡,一定浓度范围内的氟所导致的DNA损伤与细胞凋亡之间呈现明显的正相关关系。
Objective To study the relationship between DNA damage and apoptosis in human hepatocytes induced by fluorine. Methods Cultured human hepatocytes were exposed to sodium fluoride at concentrations of 40, 80 and 160μg / ml. After 12 hours, single cell gel electrophoresis (SCGE) and flow cytometry (FCM) were used to detect the effects of fluoride on human hepatocyte DNA Injury and Apoptosis. Results The DNA damage of hepatocytes in each fluorine-exposed group was significantly higher than that of the control group, and increased with the increase of fluoride dose. The percentages of hepatocyte apoptosis in medium-dose and high-dose fluoride groups were significantly higher than those in control group. At the same time, there was also a significant difference in the degree of DNA damage and apoptosis among high-dose fluoride group and low-dose fluoride group. Conclusion Fluorine can cause DNA damage in human hepatocytes and induce apoptosis. There is a clear positive correlation between DNA damage and apoptosis induced by fluorine in a certain concentration range.