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目的探讨肝缺血再灌注损伤时肝内载脂蛋白M(apoM)mRNA及血浆apoM的表达。方法将40只健康雄性SD大鼠随机分成5组(每组8只)。假手术组(对照组)进腹后仅分离十二指肠韧带,不阻断血流;IR1组灌注0.5h;IR2组缺血60min后再灌注1.0h;IR3组缺血60min后再灌注2.0h、IR4缺血60min后再灌注3.0h。在各时间点活杀大鼠,检测血浆谷丙转氨酶(ALT)水平、肝组织病理变化、血浆apoM蛋白及肝组织apoMmRNA。结果血浆ALT的水平随着灌注时间的延长而升高,肝组织损伤随着灌注时间的延长而逐渐加重。肝组织apoMmRNA的表达则先有一过性下降(IR1组),此后随着灌注时间的延长其表达明显增强。血浆apoM蛋白有相同的变化趋势,但其表达的上升有相应的滞后(IR2组)。结论在肝缺血再灌注损伤过程中,肝脏apoMmRNA的表达和血浆蛋白水平有迅速、明显的变化,提示apoM可能具有急性时相反应蛋白的特性。
Objective To investigate the expression of hepatic apolipoprotein M (apoM) mRNA and plasma apoM in liver ischemia-reperfusion injury. Methods Forty healthy male SD rats were randomly divided into five groups (n = 8). In the sham-operated group (control group), only the duodenal ligament was separated into the abdomen and the blood flow was not blocked. The IR1 group was infused for 0.5h. The IR2 group was reperfused for 1.0h after ischemia 60min and the IR3 group 60min later. h, IR4 ischemia 60min after reperfusion 3.0h. The rats were sacrificed at each time point to detect the levels of plasma ALT, pathological changes of liver tissue, plasma apoM protein and liver apoM mRNA. Results The level of plasma ALT increased with the prolongation of perfusion time, and liver injury gradually increased with the prolongation of perfusion time. The expression of apoM mRNA in liver tissue first decreased transiently (IR1 group), and then increased with the extension of perfusion time. The plasma apoM protein has the same trend, but the corresponding increase in the expression of lag (IR2 group). Conclusions During the process of hepatic ischemia-reperfusion injury, the expression of apoM mRNA and the plasma protein level in liver rapidly and obviously change, suggesting that apoM may have the characteristics of acute phase reaction protein.