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目的:观察巨噬细胞体外扩增特性及其对乳腺癌细胞MCF-7的抗肿瘤作用。方法:将巨噬细胞与乳腺癌细胞MCF-7共培养,获取初期细胞,光镜观察。以MTT法检测巨噬细胞对乳腺癌细胞MCF-7的抑制率。结果:共培养初期细胞膜完整、细胞折光性强;37℃、5%CO2、不换液、不传代培养后,细胞内颗粒增多、折光性变弱,同时死亡、破碎细胞数量增加;继续培养,细胞几乎全部死亡、破碎,培养至18天左右,体积变大、折光性变强等具有活化特征的细胞出现;采用适当方式换液后,这些折光性较强的细胞的体积迅速增大,进入分裂增殖状态;培养50天左右,细胞的分裂、增殖减缓,细胞吞噬加强,巨大细胞的数量逐渐增多。以MTT法检测巨噬细胞对乳腺癌细胞MCF-7的抑制率,在效靶比为5:1、10:1、20:1、40:1时,抑制率随着效靶比的增加而增加,成正相关。而当效靶比为100:1时,反而促进肿瘤细胞的增殖。结论:采用死亡肿瘤细胞悬液可诱导PBMC获得巨噬细胞,巨噬细胞在一定浓度的范围内可抑制肿瘤细胞的生长,而当浓度过高时反而促进肿瘤细胞的生长。
OBJECTIVE: To observe the in vitro expansion characteristics of macrophages and its antitumor effect on breast cancer cells MCF-7. Methods: Macrophages were co-cultured with breast cancer cells MCF-7, and the initial cells were obtained and observed under light microscope. The inhibitory rate of macrophages on breast cancer cells MCF-7 was detected by MTT assay. Results: At the initial stage of co-culture, the cell membrane was complete and the cell refraction was strong. At 37 ℃, 5% CO 2, no liquid change and no subculturing, the number of intracellular particles increased, the refraction became weaker and the number of broken cells increased. Almost all of the cells died, crushed, cultured to 18 days, the volume becomes larger, refractive properties of cells with activation features appear; using the appropriate way to change the liquid, these rapidly increasing refractive index of the cell volume, into Splitting proliferation state; cultured for about 50 days, cell division, proliferation slowed down, phagocytosis strengthened, the number of large cells gradually increased. The inhibitory rate of MCF-7 on macrophages was detected by MTT assay. When the ratio of target to target was 5: 1, 10: 1, 20: 1, 40: 1, Increase, into a positive correlation. When the effective target ratio of 100: 1, but to promote the proliferation of tumor cells. CONCLUSION: Macrophages can be obtained from PBMCs by using dead tumor cell suspension. Macrophages can inhibit the growth of tumor cells in a certain concentration range, but promote the growth of tumor cells when the concentration is too high.