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采用胎盘绒毛组织体外孵育模型 ,观察了胎盘阿片肽对人早期胎盘滋养层组织基质金属蛋白酶 2和 9活力的调节。结果表明 ,β 内啡肽 (10 -9mol/L)和强啡肽 (10 -10mol/L)可以显著地抑制体外孵育的人早期胎盘滋养层组织中这两种金属蛋白酶的活力 ,且其抑制效应被纳洛酮 (10 -8mol/L)所阻断 ;甲硫氨酸脑啡肽在 10 -6mol/L时显著提高二种基质金属蛋白酶的活力 ,而在 10 -8～ 10 -10 mol/L时对基质金属蛋白酶的活力无明显效应 ;亮氨酸脑啡肽则在所有试验浓度条件下对基质金属蛋白酶的活力都无显著影响。提示胎盘阿片肽可通过其受体影响基质金属蛋白酶的活力水平 ,参与胚泡着床的调控
The in vitro incubation model of placental villi was used to observe the regulation of the activity of placental opioid peptides on matrix metalloproteinase 2 and 9 in human early placental trophoblast. The results showed that both β-endorphin (10 -9 mol / L) and dynorphin (10 -10 mol / L) significantly inhibited the activity of both metalloproteases in human early placental trophoblast cells incubated in vitro, and their inhibition The effect was blocked by naloxone (10 -8 mol / L); methionine enkephalin significantly increased the activity of two matrix metalloproteases at 10 -6 mol / L, while at 10 -8 -10 -10 mol / L had no significant effect on the activity of matrix metalloproteinase; leucine enkephalin had no significant effect on the activity of matrix metalloproteinase at all tested concentrations. Prompt placental opioid peptide through its receptor affect the level of activity of matrix metalloproteinase involved in the regulation of blastocyst implantation