目的探索一种简便高纯度的早孕滋养细胞的体外培养方法。方法采用完整早孕绒毛通过胰蛋白酶联合胶原酶消化法分离,Percoll梯度分离法提纯体外培养,应用光镜HE染色和免疫细胞化学法检测细胞纯度。结果 CK-7表达阳性细胞数可以达到90%以上。结论完整绒毛消化和Percoll纯化联合可在短期内获得高纯度、高产量的滋养细胞以供后期试验,方法更加简便。 Objective To explore a simple and high purity method of in vitro culture of gestational trophoblastic cells. Methods Whole early pregnancy villus was separated by trypsin and collagenase digestion. The cells were purified by Percoll gradient centrifugation. The cell purity was detected by light microscopy and immunocytochemistry. Results CK-7 positive cells can reach more than 90%. Conclusion The combination of complete villus digestion and Percoll purification can provide high purity and high yield of trophoblast in the short term for later testing. The method is simple and convenient.