用肉眼观察和放射自显影的方法比较了9d和13d脾结节的生成。同样是能够生成脾结节的细胞,但重建照射小鼠的造血功能的潜力不同。实验结果表明,仅仅根据脾结节的生成,或者脾结节生成的先后时间,都不足以作为判定其是否为造血干细胞的唯一依据,脾结节生成细胞是在性能上不均一的细胞群体,对脾结节生成细胞的自我更新能力的实际测定,才能对其是否具有造血干细胞的基本性能做出可靠结论。 The method of visual observation and autoradiography was used to compare the formation of splenic nodules at 9 and 13 days. The same is capable of producing splenic nodules, but the potential for reconstitution in irradiated mice is different. The experimental results show that spleen nodule-producing cells are not homogeneous cell populations based solely on spleen nodule generation or splenic nodule generation time, which is not enough to determine whether it is the only basis for hematopoietic stem cells. The actual determination of the self-renewal capacity of splenic nodule-producing cells can make a reliable conclusion as to whether or not they have the basic properties of hematopoietic stem cells.