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精子发生是男性生殖中的主要过程,精原细胞的不断分裂增殖又保证了精子发生的顺利进行。随着年龄的不断增长,男性精子的数量、质量出现下降趋势。mTOR信号转导通路在细胞增殖分化中发挥着中心调控作用,因此,mTOR信号通路可能在精子发生过程中有着重要的地位。为了探明mTOR信号通路与精子发生的关系,首先,通过SD大鼠睾丸组织切片的免疫组化,发现mTOR是在生精小管的精原细胞胞浆中表达;其次,采用FQ-PCR检测mTOR mRNA在SD大鼠睾丸中的表达。结果显示,80周龄组mTOR的转录与8周龄组相比差异显著。最后利用Western blot检测出mTOR蛋白的表达及其对下游靶蛋白P70S6K的磷酸化效率均随年龄的增长逐渐下降。同时,在用雷帕霉素处理8周龄SD大鼠中,发现精子数量减少,P70S6K磷酸化效率降低并伴随生精小管萎缩和空泡化。通过这些结果,可以看出mTOR信号转导通路可能在精子发生中发挥着重要作用。
Spermatogenesis is the main process in male reproduction. The continuous division and proliferation of spermatogonia ensure the smooth progress of spermatogenesis. With the increasing age, the number of male sperm, the quality of the downward trend. The mTOR signaling pathway plays a central regulatory role in cell proliferation and differentiation, therefore, the mTOR signaling pathway may play an important role in the process of spermatogenesis. In order to explore the relationship between mTOR signaling and spermatogenesis, we first found that mTOR was expressed in the cytoplasm of spermatogonia in the seminiferous tubules by immunohistochemistry of the testicular tissue sections of SD rats. Secondly, mTOR was detected by FQ-PCR mRNA expression in testis of SD rats. The results showed that 80-week-old mTOR transcription and 8-week-old group compared to a significant difference. Finally, using Western blot to detect the expression of mTOR protein and its downstream target protein P70S6K phosphorylation efficiency decreased with age. Meanwhile, in rapamycin-treated 8-week-old SD rats, a decrease in the number of spermatozoa, a decrease in P70S6K phosphorylation efficiency, accompanied by atrophy and vacuolization of the seminiferous tubules was observed. From these results, it can be seen that the mTOR signaling pathway may play an important role in spermatogenesis.