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目的建立SPE/UPLC方法在同一条件下同时检测血中吗啡、苯丙胺类及氯胺酮。方法采用SCX 3cc(60mg)固相萃取柱萃取血中吗啡、MA、MDMA、MDA及氯胺酮,用超高效液相色谱(UPLC)-二极管阵列检测器(PDA)检测,结合保留时间和紫外光谱进行定性、定量分析,对实验各环节进行优化,并进行实际案例检测。结果吗啡、MA、MDMA、MDA、氯胺酮的固相萃取提取回收率分别为81.4%±2.51%、88.2%±2.48%、91.8%±2.03%、93.8%±1.46%、74.8%±2.27%,峰面积和质量浓度的线性关系良好(r>0.999),线性范围分别为0.08~100μg/mL、0.4~100μg/mL、0.2~75μg/mL、0.3~75μg/mL、0.4~100μg/mL,检出限分别为30pg、200pg、80pg、100pg、200pg。结论本文所建方法适用于血中吗啡、苯丙胺类、氯胺酮常见毒品的筛选及定量分析。
Objective To establish an SPE / UPLC method for the simultaneous detection of morphine, amphetamines and ketamine in the blood under the same conditions. Methods Blood samples of morphine, MA, MDMA, MDA and ketamine were extracted with SCX 3cc (60mg) solid phase extraction column and detected by UPLC - PDA with retention time and UV spectrum Qualitative and quantitative analysis, to optimize the various aspects of the experiment, and the actual case detection. Results The recovery rates of morphine, MA, MDMA, MDA and ketamine were 81.4% ± 2.51%, 88.2% ± 2.48%, 91.8% ± 2.03%, 93.8% ± 1.46%, 74.8% ± 2.27% The linear range was 0.08 ~ 100μg / mL, 0.4 ~ 100μg / mL, 0.2 ~ 75μg / mL, 0.3 ~ 75μg / mL and 0.4 ~ 100μg / mL respectively Respectively 30pg, 200pg, 80pg, 100pg, 200pg. Conclusion The method proposed in this paper is suitable for the screening and quantitative analysis of common drugs of blood morphine, amphetamines and ketamine.