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目的探索金属离子铅对K562细胞红系分化能力的影响。方法应用胎盼蓝染色排除法分析铅离子对K562细胞的活性的影响;用联苯胺染色法检测铅离子作用下K562细胞的红系分化率;利用荧光标记抗体结合流式细胞技术分析铅离子处理K562细胞的细胞表面转铁蛋白受体CD71的表达。结果铅离子(10～50μmol/L)分别作用24、48和72 h对K562细胞生长有浓度依赖性的抑制作用;铅离子(10～30μmol/L)作用48 h对氯化高铁血红素诱导的K562细胞血红蛋白合成表现出较为明显的浓度依赖性抑制;铅离子(30μmol/L)作用48 h可一定程度地上调CD71在细胞表面的表达。结论铅离子在一定的浓度作用下对K562细胞的诱导红系分化有抑制作用,为进一步应用K562细胞研究铅离子的红系血液毒性的细胞分子机制提供了一定的实验依据。
Objective To explore the effect of metal ion lead on erythroid differentiation of K562 cells. Methods The effect of lead ion on the activity of K562 cells was analyzed by trypan blue exclusion method. The erythroid differentiation rate of K562 cells was detected by benzidine staining. The effect of lead ion treatment by fluorescence labeled antibody combined with flow cytometry Expression of the cell surface transferrin receptor CD71 on K562 cells. Results Lead (10-50 μmol / L) inhibited the growth of K562 cells in a concentration-dependent manner for 24, 48 and 72 h, respectively. Lead (10-30 μmol / L) K562 cells hemoglobin synthesis showed a more significant concentration-dependent inhibition; lead ion (30μmol / L) for 48 h can up-regulate the expression of CD71 on the cell surface. Conclusions Lead ion can inhibit erythroid differentiation induced by K562 cells under certain concentration, which provides experimental basis for further application of the molecular mechanism of K562 cells in studying erythrocyte hematotoxicity of lead ions.