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目的观察冠通方含药血清对体外培养的人外周血内皮祖细胞(EPCs)数量和迁移功能的影响。方法将24只雄性Wistar大鼠随机分为冠通方组(低剂量组、中剂量组、高剂量组)和对照组,制备含药血清和空白血清。采用密度梯度离心法分离获得人外周血单个核细胞,经激光共聚焦显微镜、流式细胞仪鉴定为EPCs。加入各实验血清组培养液培养EPCs,分别作用不同时间(12,24,48,72h)后,倒置相差显微镜下观察EPCs生长情况,并分别观察EPCs的数量和迁移能力。结果与对照组相比,冠通方干预组可促进外周血EPCs扩增,并显著改善外周血EPCs的迁移能力。结论冠通方可增加EPC的数量并改善其迁移功能,这可能是冠通方防治冠心病患者经皮冠状动脉腔内成形术(PTCA)加支架植入术后再狭窄的作用机制之一。
Objective To observe the effect of Guan Tong Fang containing serum on the number and migration of human peripheral blood endothelial progenitor cells (EPCs) in vitro. Methods Twenty-four male Wistar rats were randomly divided into Guan Tong Fang group (low dose group, middle dose group, high dose group) and control group, and drug-containing serum and blank serum were prepared. Human peripheral blood mononuclear cells were isolated by density gradient centrifugation and identified by laser confocal microscopy and flow cytometry. EPCs were cultured in the culture medium of each experimental group, the growth of EPCs was observed under inverted phase contrast microscope at different time (12,24,48,72h), and the number and migration ability of EPCs were observed. Results Compared with the control group, Guan Tong Fang intervention group can promote the expansion of EPCs in peripheral blood and significantly improve the migration ability of EPCs in peripheral blood. Conclusion GuanTongFang can increase the number of EPCs and improve their migration function, which may be one of the mechanisms of GuanTongFang in the prevention and treatment of restenosis after percutaneous transluminal coronary angioplasty (PTCA) and stent implantation in patients with coronary heart disease.