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目的 :检测 10 4例鼻咽癌活检组织和 8例鼻咽粘膜慢性炎症组织中p5 3蛋白的表达。方法 :采用Western印迹法结合免疫沉淀技术。结果 :10 4例鼻咽癌活检组织中有 12例出现突变型p5 3蛋白表达 ,突变检出率为 11.5 4% (12 10 4) ;8例鼻咽慢性炎症活检组织中未见p5 3蛋白的异常表达 (0 8) ;鼻咽癌病人中人IgA VCA抗体滴度≥ 1∶16 0者 ,p5 3蛋白突变检出率为11.80 % (11 93) ,IgA VCA抗体滴度≤ 1∶80者 ,p5 3蛋白突变检出率为 9.10 % (1 11) ,IgA EA抗体滴度阳性者 ,p5 3蛋白突变检出率为 14.10 % (11 78) ,IgA EA抗体滴度阴性者 ,p5 3蛋白突变检出率为 3.80 % (1 2 6 ) ;12例p5 3蛋白异常表达的鼻咽癌病人中有 2例复发。结论 :突变型p5 3基因产物的表达与鼻咽癌EB病毒早期抗原有关 ,p5 3基因在鼻咽癌中可能发挥一定的作用
OBJECTIVE: To detect the expression of p5 3 protein in 104 nasopharyngeal carcinoma biopsies and 8 chronic nasopharyngeal mucosal inflammatory tissues. Methods: Western blotting and immunoprecipitation were used. Results: The mutation type p5 3 protein was found in 12 out of 104 cases of nasopharyngeal carcinoma biopsy tissues, the mutation detection rate was 11.5 4% (12 10 4). No p5 3 protein was found in 8 chronic nasopharyngeal biopsy tissues (0 8). The detection rate of p5 3 protein mutation was 11.80% (11 93) in patients with nasopharyngeal carcinoma, the titer of IgA VCA antibody was 1:80 , The mutation rate of p5 3 protein was 9.10% (11), the positive rate of IgA EA antibody titer was 14.10% (11 78), the mutation rate of p5 3 protein was negative, p5 3 The detection rate of protein mutation was 3.80% (12 6). Twelve cases of nasopharyngeal carcinoma patients with abnormal expression of p5 3 protein recurred. CONCLUSIONS: The expression of mutant p5 3 gene product is related to early EBV antigen in NPC, and p5 3 gene may play a role in NPC