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目的 探讨金属硫蛋白 (metallothionein ,MT)在肝细胞癌 (hepatocellularcarcinoma,HCC)组织中、癌旁组织和正常肝组织中的表达及其临床意义。方法 对 30例HCC病人的癌组织、10例癌旁组织和 10例正常肝组织标本进行免疫组化染色 ,观察MT的表达状况 ,分析MT在HCC中的表达与癌旁组织、正常肝组织、细胞分化和预后等的关系。结果 正常肝组织有很强而稳定的MT染色 ;癌旁组织 (包括肝硬化结节 )有较高的MT着色 ;癌组织中MT染色很弱 ,甚至无MT染色。相互比较有显著性差异 (P <0 0 5 )。MT在肝细胞癌组织中的表达与生存率之间存在着正相关关系(P <0 0 5 )。结论 MT在正常肝组织、癌旁组织和肝细胞癌组织中的表达呈递减趋势 ,肝细胞癌组织中MT表达与癌细胞分化无关 ,而与瘤体组织坏死及肝切除术后病人生存状况相关。
Objective To investigate the expression and clinical significance of metallothionein (MT) in hepatocellularcarcinoma (HCC) tissues, adjacent noncancerous tissues and normal liver tissues. Methods Immunohistochemical staining was performed on 30 cases of HCC patients with cancer tissues, 10 cases of paracancerous tissues and 10 cases of normal liver tissues. The expression of MT was observed. The expression of MT in HCC tissues was compared with that of paracancerous tissues, normal liver tissues, Cell differentiation and prognosis of the relationship. Results The normal liver tissue had a strong and stable MT staining. The paracancerous tissues (including cirrhosis nodules) had higher MT staining; MT staining in cancer tissues was very weak, even without MT staining. There was significant difference between them (P <0.05). There was a positive correlation between the expression of MT in hepatocellular carcinoma and the survival rate (P <0.05). Conclusions The expression of MT in normal liver tissue, paracancerous tissue and hepatocellular carcinoma has a decreasing trend. The expression of MT in hepatocellular carcinoma has nothing to do with the differentiation of cancer cells, but is related to the tumor tissue necrosis and survival after liver resection .