论文部分内容阅读
目的:研究抗生素相关性腹泻与肠道真菌菌群多样性的关系。方法:将BALB/C雄性小鼠分为空白对照组、生理盐水组、实验组和恢复组。实验组给予头孢拉啶联合庆大霉素腹腔注射,2次/d,收集小鼠第3、5、7、14和21天的粪便,采用变性梯度凝胶电泳实验方法分析真菌菌群多样性变化。结果:与空白对照组比,实验组小鼠真菌菌群多样性明显增加(21.15±2.66 vs 15.5±0.57,P<0.05)。恢复组(停药7 d)小鼠真菌菌群多样性明显降低,与空白对照组比无显著性差异(14.5±3.08 vs 15.5±0.57,P>0.05)。结论:抗生素相关性腹泻与肠道真菌菌群多样性有关,且以其多样性增加为主。抗生素可能对肠道真菌菌群的变化有影响。
Objective: To study the relationship between antibiotic-associated diarrhea and intestinal flora diversity. Methods: BALB / C male mice were divided into blank control group, normal saline group, experimental group and recovery group. The experimental group was given cefradine and gentamycin intraperitoneal injection, 2 times / d, mice were collected on the 3rd, 5th, 7th, 14th and 21st days of stool, denaturing gradient gel electrophoresis test method analysis of fungal flora diversity Variety. Results: Compared with the blank control group, the diversity of fungi in experimental group was significantly increased (21.15 ± 2.66 vs 15.5 ± 0.57, P <0.05). The fungal flora diversity in the recovery group (7 days after drug withdrawal) was significantly lower than that in the blank control group (14.5 ± 3.08 vs 15.5 ± 0.57, P> 0.05). Conclusion: The antibiotic-associated diarrhea is related to the diversity of intestinal fungal flora, with the increase of its diversity. Antibiotics may have effects on changes in intestinal flora.