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目的:本研究旨在探索紫杉醇、顺铂诱导卵巢癌细胞凋亡后能否具有较强的免疫原性,并可以被抗原提呈细胞交叉提呈并促进免疫应答。方法:用巨噬细胞-集落刺激因子(GM-CSF)和白介素-4(IL-4)刺激人外周血单核细胞分化诱导为树突状细胞(DC),6d后将DC和经紫杉醇联合顺铂在体外诱导发生凋亡的人卵巢癌细胞株共同培养,并以冻融细胞共培养DC组和单独DC组作对照,共培养4h后,进行激光扫描共聚焦显微镜观察DC细胞对凋亡细胞的吞噬作用,同时以流式细胞术(FCM)检测不同培养组DC的分化和成熟程度。结果:紫杉醇、顺铂诱导的凋亡卵巢癌细胞可被DC有效吞噬,与对照组相比,凋亡组DCs的表达及成熟度均明显增高,并具有统计学意义(P(0.05)。结论:紫杉醇、顺铂诱导的凋亡卵巢癌细胞具有较强的免疫原性,可以促进DC的分化和成熟。
OBJECTIVE: This study aimed to explore whether paclitaxel can induce ovarian cancer cells to apoptosis after ovarian cancer cells have strong immunogenicity, and can be cross-presented by antigen-presenting cells and promote immune response. Methods: Dendritic cells (DCs) were induced by monocyte-macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) Cisplatin co-cultured human ovarian cancer cell line induced apoptosis in vitro, and co-cultured with freeze-thawed cells DC group and DC group as a control, co-cultured 4h, the laser scanning confocal microscope DC apoptosis The phagocytosis of cells was also observed by flow cytometry (FCM) to detect the differentiation and maturation of DC in different culture groups. Results: Paclitaxel and cisplatin-induced apoptosis of ovarian cancer cells can be effectively phagocytosed by DC, and the expression and maturation of DCs in apoptotic group were significantly increased compared with the control group (P <0.05) .Conclusion : Paclitaxel, cisplatin-induced apoptosis of ovarian cancer cells with strong immunogenicity, can promote the differentiation and maturation of DC.