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目的 了解消化性溃疡和慢性胃炎患者感染的幽门螺杆菌 (Hp)cagA vacA优势基因型及不同基因型Hp感染、混合感染与疾病的关系。 方法 选择胃窦、胃体双份活检标本均培养出Hp的 42例慢性胃炎 (CG)和 3 6例消化性溃疡 (PU )患者作为研究对象 ,采用聚合酶链反应 (PCR)检测156份Hp分离株的cagA基因、vacA基因的信号区 (s)和中间区 (m )亚型 ,分析Hp基因型及多株Hp混合感染在CG和PU中的分布。结果 胃窦标本cagA基因的检测中 78例患者中有 75例 (96.2 % )为cagA阳性 ,相应的胃体标本中 ,76例患者 (97.4% )为cagA阳性 ,有 1例 (1.3 % )患者胃窦、胃体检出cagA状态不一的Hp混合菌株。在胃窦标本的vacA基因分型中 ,s1a m1、s1a m2、s1a m1b、s1a m1b m2 4种vacA基因型在 78例患者中所占比例分别为 6.4% (5 78)、55.1% (43 78)、2 6.9% (2 1 78)和 1.3 % (1 78) ,多株混合感染为 3 .8% (3 78) ;而相应的胃体标本中 ,前述四种vacA基因型所占比例依次为 6.4% (5 78)、53 .8% (42 78)、2 5.6% (2 0 78)和 3 .8% (3 78) ,多株混合感染为 5.1% (4 78)。cagA+ s1a m2和cagA+ s1a m1b在胃窦标本中占 51.3 % (40 78)和 2 6.9% (2 1 78) ,而在相应的胃体标本中占 52 .6% (41 78)和 2 5.6% (2 0 78)。少量胃窦、胃体
Objective To investigate the dominant genotypes of cagA vacA in Helicobacter pylori (Hp) and Hp infection of different genotypes in patients with peptic ulcer and chronic gastritis and the relationship between the mixed infection and the disease. Methods Forty-two chronic gastritis (CG) and 36 peptic ulcer (PU) patients with Hp were cultured in gastric biopsy specimens of gastric antrum and gastric body, and 156 Hp samples were detected by polymerase chain reaction (PCR) The cagA gene of the isolate, the signal region (s) of the vacA gene and the subtype of the middle region (m) were used to analyze the distribution of Hp genotype and multiple Hp co-infections in CG and PU. Results In the gastric specimens, 75 (96.2%) of 78 patients were positive for cagA. Of the 76 samples (97.4%), cagA was positive in 1 of the 78 samples (1%) Gastric antrum, gastric body detected cagA mixed state of Hp strains. In the vacA genotyping of gastric specimens, the four vacA genotypes of s1a m1, s1a m2, s1a m1b and s1a m1b m2 accounted for 6.4% (78%), 55.1% (43.78% ), 2 6.9% (2 1 78) and 1.3% (1 78) respectively. The infection rate of multiple strains was 3.8% (3 78). The proportion of the above four vacA genotypes was Were 6.4% (5 78), 53.8% (42 78), 2 5.6% (20 78) and 3.8% (3 78), respectively, and the infection rate of multiple strains was 5.1% (4 78). The cagA + s1a m2 and cagA + s1a m1b accounted for 51.3% (40 78) and 2 6.9% (21 78) in gastric specimens, while 52.6% (41 78) and 2 5.6% (2 0 78). A small amount of gastric antrum, stomach