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目的:利用响应面分析法优化纤维素酶和果胶酶复合水解丹参提取得到丹酚酸B的工艺。方法:在单因素试验基础上选取因素与水平,利用SAS分析软件以丹酚酸B得率为相应值,采用四因素三水平的响应面分析法,以获得多元二次线性回归方程选取较优工艺,并进行预测。结果:确定最优工艺条件为纤维素酶与果胶酶的配比为2∶1,复合酶量为68 U,酶解温度38.8℃,酶解pH 3.8,酶解时间0.9 h,提取预测值与理论值的相对误差为1.1%。结论:响应面法优化纤维素酶和果胶酶复合水解丹参提取得到丹酚酸B的工艺,方法简便,可预测性较优。
OBJECTIVE: To optimize the process of hydrolysis of Salvia miltiorrhiza B with extraction of cellulose and pectinase by response surface methodology. Methods: Factors and levels were selected on the basis of single-factor test. SAS analysis software was used to obtain the yield of salvianolic acid B as the corresponding value, and the response surface analysis of four factors and three levels was used to obtain the optimal selection of multivariate quadratic regression equation Craft and make predictions. Results: The optimum conditions were as follows: the ratio of cellulase to pectinase was 2:1, the amount of complex enzyme was 68 U, the enzymolysis temperature was 38.8 ℃, the pH was 3.8 and the enzymolysis time was 0.9 h, The relative error from the theoretical value is 1.1%. CONCLUSION: The process of extracting salvianolic acid B from the hydrolysis of Salvia miltiorrhiza by cellulase and pectinase is optimized by response surface methodology. The method is simple and predictable.