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目的探索染色体8q24区域(rs620861C、rs1447295A)和17q24区域(rs185996,G)风险等位基因与北方中国人前列腺癌的关联。了解其基因型与前列腺癌患者表型(年龄、肿瘤分期、PSA水平、Gl-eason评分、有无良性前列腺肥大)的关联,分析基因之间的交互作用。方法采用病例-对照设计,收集前列腺癌患者临床表型信息;采用PCR-HRM和测序技术对各位点进行了分型,并对基因型在病例组和正常对照组中的分布进行了年龄匹配的病例-对照间比较(病例组:124人,对照组:138人)及基因交互作用分析;对病例组中基因型在不同临床表型中的分布进行了比较。结果在病例-对照之间三个风险位点的基因型和等位基因频率分布差异未见有统计学意义;在病例组中各位点与前列腺癌表型之间未见有显著性关联;未能检测到三个风险位点之间有交互作用。结论染色体8q24区域(rs620861C、rs1447295A)和17q24区域(rs185996G)可能与北方中国人患前列腺癌的遗传风险无关联。
Objective To explore the association of the risk alleles of chromosome 8q24 (rs620861C, rs1447295A) and 17q24 (rs185996, G) with northern Chinese prostate cancer. To understand its genotype and prostate cancer phenotype (age, tumor stage, PSA level, Gl-eason score, with or without benign prostatic hypertrophy) association, analysis of gene interactions. Methods The case-control design was used to collect clinical phenotype information of patients with prostate cancer. PCR-HRM and sequencing were used to classify each loci and the age-matched genotype distribution in case group and normal control group Case-control comparisons (case group: 124, control group: 138) and analysis of gene interaction; distribution of genotypes in different clinical phenotypes in the case group were compared. Results There were no significant differences in genotype and allele frequency distribution between the three risk sites in the case-control group; no significant association was found between the loci and the prostate cancer phenotype in the case group; Interaction between the three risk sites can be detected. Conclusion The chromosome 8q24 region (rs620861C, rs1447295A) and 17q24 region (rs185996G) may not be associated with the genetic risk of prostate cancer in northern China.