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目的研究化疗联合重组人粒细胞集落刺激因子(rhG-CSF)动员自体外周血干细胞的效果。方法对10例急性白血病和恶性实体瘤患者采用动员方案为化疗+重组人粒细胞集落刺激因子。10例患者中,急性淋巴细胞白血病(ALL)1例,急性非淋巴细胞白血病(ANLL)2例,非霍奇金淋巴瘤(NHL)4例,霍奇金淋巴瘤(HD)1例,乳腺癌1例,恶性黑色素瘤1例。主要动员方案为CTV方案:环磷酰胺(CTX)1200～1500mg/m2,鬼臼噻吩甙(Vm-26)400mg/m2,分3天用,长春花碱酰胺(VDS)3mg/m2。于联合化疗后WBC降至最低并开始回升时予rhG-CSF5μg/kg,1次/天,皮下注射,当WBC≥5.0×109/L时开始用血细胞分离机采集。结果1例采集1次,9例采集2次,获得MNC为(5.62±2.25)×108/kg,CD34+细胞为(13.26±6.45)×106/kg,CFU-GM为(2.33±1.02)×105/kg。达到目标采集量单个核细胞≥4×108/kg10例中8例(80%),CD34+细胞≥2×106/kg10例中10例(100%),CFU-GM≥2×105/kg10例中7例(70%)。1次采集后8例(80%),2次采集后10例(100%),患者达到了CD34+细胞数目标采集量。结论化疗CTV方案联合rhG-CSF动员可采集到足够数量的外周血干细胞。
Objective To study the effect of chemotherapy combined with recombinant human granulocyte-colony stimulating factor (rhG-CSF) on peripheral blood stem cells mobilization. Methods Ten patients with acute leukemia and solid tumor patients were mobilized for chemotherapy + recombinant human granulocyte colony-stimulating factor. Among the 10 patients, 1 had acute lymphoblastic leukemia (ALL), 2 had acute non-lymphocytic leukemia (ANLL), 4 had non-Hodgkin’s lymphoma (NHL), 1 had Hodgkin’s lymphoma (HD) 1 case of cancer, 1 case of malignant melanoma. The main regimen was CTV regimen: cyclophosphamide (CTX) 1200 ~ 1500mg / m2, Diazaplan (Vm-26) 400mg / m2, for 3 days, vinblastine 3mg / m2. When the WBC was reduced to a minimum after the combination chemotherapy and started to rise, rhG-CSF (5μg / kg, once daily, subcutaneously) was injected subcutaneously. When the WBC was greater than or equal to 5.0 × 109 / L, the blood was collected by a hemocyte separator. Results One case was collected and the other 9 cases were collected twice. The MNC was 5.62 ± 2.25 × 108 / kg, the CD34 + cells was (13.26 ± 6.45) × 106 / kg and the CFU-GM was (2.33 ± 1.02) × 105 / kg. The target collection rate was 8 cases (80%) in 10 cases of mononuclear cells≥4 × 108 / kg, 10 cases (100%) in 10 cases of CD34 + cells≥2 × 106 / kg and CFU-GM≥2 × 105 / kg 7 cases (70%). 8 cases (80%) after one acquisition and 10 cases (100%) after two acquisitions. The patients achieved the target collection of CD34 + cells. Conclusion Chemotherapy CTV regimen combined with rhG-CSF mobilization can collect a sufficient number of peripheral blood stem cells.