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目的了解福州市食物中毒沙门菌血清型、耐药性和毒力基因分布情况,为预防该菌引起的食物中毒提供实验依据。方法采用国家标准检测方法进行沙门菌检验,测定抗菌药的最低抑菌浓度(MIC)值进行耐药性研究,PCR扩增检测毒力基因。结果 18株不同来源的沙门菌中,肠炎沙门菌15株,占83.3%,不同来源的沙门菌血清型分布差异无统计学意义(P>0.05)。18株不同来源沙门菌多重耐药率为100.0%;红霉素、阿奇霉素、庆大霉素、头孢唑啉、头孢西丁耐药率均为100.0%,氨苄西林(AMP)、氨苄西林/舒巴坦、四环素、氯霉素、萘啶酸的耐药率均超过50%,其差异无统计学意义(P>0.05)。7种毒力因子(avrA、ssaQ、mgtC、siiD、sopB、spvC、bcfC)的携带率为100.0%,sodC1的携带率为94.4%,sopE1的携带率为88.9%。结论福州市人源性和食源性沙门菌中,肠炎沙门菌是主要血清型,分离株携带多种毒力因子,致病性强,多重耐药现象均普遍存在。
Objective To understand the serotypes, drug resistance and virulence genes of Salmonella typhimurium in Fuzhou and to provide experimental evidence for preventing food poisoning caused by this bacterium. Methods Salmonella test was carried out by the national standard test method, and the minimum inhibitory concentration (MIC) value of antimicrobial agents was determined for drug resistance study. Virulence genes were detected by PCR amplification. Results Among the 18 Salmonella isolates, 15 were Salmonella enteritidis, accounting for 83.3%. There was no significant difference in the distribution of Salmonella serotypes among different origins (P> 0.05). The multi-drug resistance rates of Salmonella from 18 different sources were 100.0%. The rates of resistance to erythromycin, azithromycin, gentamycin, cefazolin and cefoxitin were 100.0%, and ampicillin (AMP) and ampicillin / The resistance rates of tuberculosis, tetracycline, chloramphenicol and nalidixic acid all exceeded 50%, with no significant difference (P> 0.05). The carrying rate of seven virulence factors (avrA, ssaQ, mgtC, siiD, sopB, spvC and bcfC) was 100.0%, sodC1 carrying rate was 94.4%, and sopE1 carrying rate 88.9%. Conclusions Salmonella enteritidis is the major serotype in both human and food-borne Salmonella in Fuzhou. The isolates harbor multiple virulence factors, which are highly pathogenic and multi-drug resistant.