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[目的]观察线粒体部分敲除对氡染毒人支气管上皮细胞损伤效应的影响。[方法]分别对线粒体DNA部分敲除的人支气管上皮细胞(ρ-HBE细胞)和未经敲除的ρ+HBE细胞暴露20 000 Bq/m3的氡及氡子体,观察两种受试细胞第10代(低剂量组,ρ+HBE-Rn10和ρ-HBE-Rn10)及第30代(高剂量组,ρ+HBE-Rn30和ρ-HBE-Rn30)细胞的增殖能力、细胞周期以及活性氧(ROS)水平的变化。[结果]氡染毒后,线粒体部分敲除后高、低剂量组(ρ-HBE-Rn30,ρ-HBE-Rn10)的细胞克隆形成率(PE)和细胞相对存活分数(SF)均高于敲除前(P<0.05);敲除前高剂量组(ρ+HBE-Rn30)的PE和SF均低于低剂量组(ρ+HBE-Rn10)(P<0.05),敲除后则高于低剂量组(P<0.05)。低剂量组ρ+HBE细胞中的S期细胞减少,G2/M期细胞增加,高剂量组的S期细胞增加,G1期细胞减少;各染毒剂量的ρ+HBE细胞和ρ-HBE细胞,ROS水平均升高(P<0.05)。[结论]经氡染毒后,ρ-HBE细胞在生长速度、细胞周期、ROS产生量等生物学指标上与ρ+HBE细胞有明显改变,表明氡所产生的细胞损伤效应与线粒体的结构和功能障碍密切相关。
[Objective] To observe the effect of partial mitochondrial knockdown on the injury of bronchial epithelial cells exposed to radon. [Methods] The radon and radon progeny of 20 000 Bq / m3 were exposed to partially knocked-out human bronchial epithelial cells (ρ-HBE cells) and untreated ρ + HBE cells, respectively. Two kinds of test cells Proliferation, cell cycle and activity of the 10th generation (low dose group, ρ + HBE-Rn10 and ρ-HBE-Rn10) and 30th generation (high dose group, ρ + HBE-Rn30 and ρ-HBE-Rn30) Changes in oxygen (ROS) levels. [Result] After radon exposure, the cell colony formation rate (PE) and relative cell viability (SF) of high and low dose groups (ρ-HBE-Rn30 and ρ-HBE-Rn10) The levels of PE and SF in high dose group (ρ + HBE-Rn30) before knocking were lower than those in low dose group (ρ + HBE-Rn10) (P <0.05) In low dose group (P <0.05). In the low dose group, the number of S phase cells, the G2 / M phase cells in the ρ + HBE cells increased, the S phase cells in the high dose group increased, and the cells in the G1 phase decreased. The ρ + HBE cells and ρ-HBE cells, ROS levels were increased (P <0.05). [Conclusion] The ρ-HBE cells changed significantly with the ρ + HBE cells in the biological indexes such as growth rate, cell cycle and ROS production after radon exposure, indicating that the cell damage effect caused by radon is related to the structure of mitochondria and Dysfunction is closely related.