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目的通过西吡氯铵含片对口源性口臭致臭菌和致臭底物的实验研究,探讨西吡氯铵含片对口源性口臭治疗的效果。方法通过连续稀释法检测西吡氯铵对致臭菌牙龈卟啉单胞菌(P.gingivalis,Pg)、中间普雷沃菌(P.intermedius,Pi)、具核梭杆菌(F.subsp nucleatum,Fn)和口腔正常有益菌唾液链球菌(Streptococcus salivarius,Ss)的最低抑菌浓度(minimal Inhibitory Concentration,MIC);并检测西吡氯铵对3种致臭菌分别培养4 h和8 h后的挥发性硫化物(volatile sulfur compounds,VSCs)水平的影响;进一步通过半胱氨酸激发模拟口臭实验,检测西吡氯铵含片对激发的VSCs的抑制率和持续时间。结果西吡氯铵对所有实验菌具有抑菌能力,但对唾液链球菌MIC值均高于其对致臭菌的MIC值;1%西吡氯铵与1%氯己定在作用Pg、Pi和Fn 4 h时,抑制作用相当(P>0.05),但在作用8 h时,抑制作用西吡氯铵低于氯己定(P<0.05);半胱氨酸激发模拟口臭实验结果显示,西吡氯铵含片在30~120 min内对VSCs水平的抑制率与1%聚维酮碘漱口液相当(P>0.05),在60~120 min内高于西吡氯铵漱口液(P<0.05)。且西吡氯铵含片下调半胱氨酸激发的VSCs水平的持续时间为235 min,长于西吡氯铵漱口液,差异有统计学意义(P<0.05)。结论西吡氯铵含片能有效抑制口腔致臭菌,对治疗口源性口臭有良好的效果。
Objective To investigate the effect of cetylpyridinium chloride tablets on the treatment of oral halitosis by the experimental study of ciclopirox sodium buccal tablets on oral and oral malodor. Methods The effects of cetylpyridinium chloride on the growth of P. gingivalis (Pg), P. intermedius (Pi), F.subsp nucleatum , Fn), and minimal inhibitory concentration (MIC) of normal oral bacteria, Streptococcus salivarius (Ss). The effects of cetylpyridinium chloride on the three kinds of odorous bacteria after 4 h and 8 h Of volatile sulfur compounds (VSCs). The inhibitory rate and duration of cetylpyridinium chloride-loaded VSCs were further tested by cysteine-induced simulated halitosis. Results Cetylpyridinium had antibacterial activity against all the tested bacteria, but the MIC values of Streptococcus salivarius were all higher than those of the MIC for odorous bacteria. The effect of 1% Cetylpyridinium chloride and 1% chlorhexidine on Pg, Pi (P> 0.05). However, the inhibitory effect was less than that of chlorhexidine (P <0.05) at 8 h. The results of cysteine challenge simulating halitosis showed that, The inhibitory rate of cetylpyridinium chloride tablets to VSCs was 30 ~ 120 min (P> 0.05), which was higher than that of cetylpyridinium chloride in 60 ~ 120 min (P <0.05). The duration of cysteine-induced VSCs release by cetylpyridinium tablets was 235 min, which was longer than that of cetylpyridinium chloride (P <0.05). Conclusion Cetylpyridone tablets can effectively inhibit odoral bacteria in oral cavity, which has a good effect on the treatment of oral bad breath.