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为了研究硫代反义寡核苷酸（ＡＳＯＮ）的抗乙型肝炎病毒（ＨＢＶ）作用，以２．２．１５细胞为靶细胞，在ＨＢＶＳ基因起始区设计了１段ＡＳＯＮ，用酶联免疫吸附法检测了作用细胞的ＨＢＶ（ＨＢｓＡｅ）的分泌情况。结果显示，ＡＳＯＮ在每天给予２ｕｍｏｌ／Ｌ的浓度情况下能抑制８８％的ＨＢｓＡｇ产生，无关序列的寡核苷酸无明显抑制作用，同时未见ＡＳＯＮ的细胞毒作用。提示ＡＳＯＮ可能成为抗ＨＢＶ治疗的新手段。
In order to investigate the anti-hepatitis B virus (HBV) effect of antisense oligonucleotide of ASON (ASON), 2.2.15 cells were used as target cells and ASON was designed in the initiation region of HBVS gene. The secretion of HBV (HBsAe) from the effector cells was examined by immunoadsorption assay. The results showed that ASON could inhibit the production of HBsAg by 88% at the concentration of 2umol / L every day, while the oligonucleotide with unrelated sequence had no obvious inhibitory effect, while no ASON cytotoxicity was observed. Tip ASON may become a new anti-HBV treatment.