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目的:研究吉西他滨对人非小细胞肺癌A549及H1299细胞增殖及凋亡的影响。方法:应用MTT法及流式细胞技术测定不同浓度的吉西他滨对A549及H1299细胞体外增殖的抑制作用。结果:(1)MTT法检测不同浓度(1.25、2.5、5、10、20、40、60μg/ml)吉西他滨分别作用于A549及H1299细胞24、48、72h后,其对A549和H1299细胞的抑制作用逐渐增强,且具有一定的剂量和时间依赖性,但是随着时间的延长,吉西他滨对A549具有药物耐受性,而对H1299具有药物敏感性;(2)A549及H1299细胞随着培养时间的延长,细胞生长逐渐进入对数期,后进入平台期;(3)倒置显微镜下观察发现,A549及H1299对照组细胞贴壁生长,生长状态良好,形态大多数为多角形、或梭形,贴壁牢固,细胞间连接紧密。随着药物作用时间的增加,细胞逐渐皱缩、坏死、碎裂;(4)流式细胞仪检测不同浓度的吉西他滨(0、2.5、5、10、20、40μg/ml)对A549及H1299细胞凋亡具有明显的剂量依赖关系。结论:吉西他滨对A549及H1299细胞增殖有较强的抑制作用;呈现一定的浓度抑制作用和时间抑制作用。随着时间的延长,吉西他滨对A549细胞耐药,而对H1299细胞敏感。
Objective: To study the effects of gemcitabine on proliferation and apoptosis of human non-small cell lung cancer A549 and H1299 cells. Methods: MTT assay and flow cytometry were used to determine the inhibitory effect of different concentrations of gemcitabine on A549 and H1299 cell proliferation in vitro. Results: (1) MTT assay was used to detect the inhibitory effect of gemcitabine at different concentrations (1.25,2.5,5,10,20,40,60μg / ml) on A549 and H1299 cells at 24,48,72h With increasing dose and time dependence, but with the extension of time, gemcitabine has drug resistance to A549 and has drug sensitivity to H1299; (2) With the incubation time of A549 and H1299 (3) Observed by inverted microscope, A549 and H1299 control cells adherent growth, good growth state, the majority of morphological polygons, or fusiform, affixed to the plateau Wall solid, close connection between cells. (4) Flow cytometry was used to detect the effect of different concentrations of gemcitabine (0,2.5,5,10,20,40μg / ml) on A549 and H1299 cells Apoptosis has a significant dose-dependent relationship. CONCLUSION: Gemcitabine can inhibit the proliferation of A549 and H1299 cells in a dose-dependent and time-dependent manner. As time goes by, gemcitabine is resistant to A549 cells and sensitive to H1299 cells.