研究高效液相色谱法在水苏糖品质控制中的应用。比较两种色谱柱同一时间对同一水苏糖样品的分析,氨基柱比ODS柱具有更好的精密度、重现性和更稳定的日间变异性。面积归一化法简单,快速,可以初步判断水苏糖产品的相对含量。外标法可以在任何情况下准确定量样品水苏糖含量,排除没有峰响应物质的干扰,适用范围更广,在产品质量控制方面更准确。 Study on the Application of High Performance Liquid Chromatography in Stachyose Quality Control. Comparing the analysis of the same stachyose sample at the same time on both columns, the amino column has better precision, reproducibility and a more stable day-to-day variability than the ODS column. Area normalization method is simple and fast, you can initially determine the relative content of stachyose products. The external standard method can accurately quantify the stachyose content of the sample in any case, eliminate the interference of no peak response substance, have a wider scope of application and are more accurate in product quality control.