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目的通过探讨重庆地区慢性HBV感染者的HLA-A超型等位基因分布与健康志愿者之间的差异性,分析得出重庆地区慢性HBV感染者的易感HLA-A超型等位基因。方法应用聚合酶链反应/序列特异性引物技术(PCR-SSP)对健康人以及慢性HBV感染者的HLA-A等位基因多态性进行检测,分析不同超型中的各HLA-A位点等位基因在健康志愿者及慢性HBV感染者中人群分布频率以及等位基因频率。结果在重庆地区健康志愿者中,HLA-A3超型等位基因的表型频率(70.5%)以及等位基因频率(44.3%)均高于HLA-A2超型(45.5%;25.0%)及其它超型(P<0.01);但是,在慢性HBV感染者中,HLA-A2超型等位基因的表型频率(65.3%)及等位基因频率(36.7%)均高于HLA-A3超型(57.1%;32.7%)及其他超型等位基因(P<0.01)。结论重庆地区,HLA-A2超型等位基因与慢性HBV感染相关;HLA-A2超型等位基因可能是慢性HBV感染的易感基因,HLA-A3超型可能是HBV感染慢性化的保护性基因。
Objective To explore the differences between HLA-A super alleles in chronic HBV carriers and healthy volunteers in Chongqing, and to analyze the susceptible HLA-A super alleles in chronic HBV carriers in Chongqing. Methods Polymorphisms of HLA-A alleles in healthy individuals and patients with chronic HBV infection were detected by polymerase chain reaction / sequence specific primer (PCR-SSP), and the HLA-A alleles Population distribution frequency and allele frequency of alleles in healthy volunteers and chronic HBV infected persons. Results The phenotypic frequency (70.5%) and allele frequency (44.3%) of HLA-A3 supertype alleles in healthy volunteers in Chongqing were higher than that of HLA-A2 supertype (45.5%; 25.0%) and (P <0.01). However, in patients with chronic HBV infection, the phenotypic frequency (65.3%) and allele frequency (36.7%) of HLA-A2 supertype alleles were higher than that of HLA-A3 super-alleles Type (57.1%; 32.7%) and other supertype alleles (P <0.01). Conclusion HLA-A2 supertype alleles are associated with chronic HBV infection in Chongqing. HLA-A2 supertype alleles may be susceptible genes of chronic HBV infection. HLA-A3 supertype may be a chronic protective factor of HBV infection gene.