[目的]研究小麦种子中主要水解酶同工酶的特性。[方法]采用聚丙烯酰胺凝胶(PAGE)电泳技术,分别对萌发过程中小麦种子中胚芽、胚乳和胚根三大组织部位存在的淀粉酶、酯酶、蛋白水解酶的同工酶图谱进行了研究,[结果]实验结果表明,在小麦种子胚乳中的淀粉酶、酯酶同工酶变化较明显,而蛋白水解酶变化较大的则是在胚芽和胚根当中。[结论]该研究为今后有效提高作物种子的萌发率提供了基础资料。 [Objective] The research aimed to study the characteristics of major hydrolase isozymes in wheat seeds. [Method] The isozyme patterns of amylase, esterase and proteolytic enzyme in the three tissues of germ, endosperm and radicle in wheat seeds during germination were analyzed by polyacrylamide gel electrophoresis (PAGE) [Result] The experimental results showed that the changes of amylase and esterase isozyme in wheat endosperm were more obvious, while the changes of proteolytic enzyme were more in the germ and radicle. [Conclusion] This study provided the basic information for effectively increasing the germination rate of crop seeds in the future.