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目的:研究8-溴-7-甲氧基白杨素增强三氧化二砷(Arsenic Trioxide,ATO)对急性单核细胞白血病U937细胞毒性作用。方法:MTT比色法检测细胞毒性,流式细胞仪(FCM)测定细胞凋亡率,Western blot分析Bax和Bcl-XL蛋白表达。结果:2.0?M BrMC和ATO联合处理U937细胞的毒性作用显著高于单用相同浓度BrMC或ATO(P<0.05)。在BrMC和ATO联合作用下,U937细胞凋亡率、Bax蛋白表达显著增高,Bcl-XL蛋白表达显著降低。结论:BrMC具有增强ATO对白血病U937细胞毒性作用,其作用机制涉及上调Bax/Bcl-XL比值促进细胞凋亡。
OBJECTIVE: To study the cytotoxicity of 8-bromo-7-methoxy-chrysin to enhance the cytotoxicity of Arsenic trioxide (ATO) on acute monocytic leukemia U937 cells. Methods: The cytotoxicity was detected by MTT colorimetric assay. The apoptosis rate was determined by flow cytometry (FCM). The protein expression of Bax and Bcl-XL was analyzed by Western blot. Results: The toxic effect of 2.0? M BrMC and ATO combined treatment on U937 cells was significantly higher than that of BrMC or ATO alone (P <0.05). Under the combined action of BrMC and ATO, the apoptosis rate of U937 cells, Bax protein expression was significantly increased, Bcl-XL protein expression was significantly reduced. Conclusion: BrMC can enhance the cytotoxicity of ATO on U937 leukemia cells. Its mechanism may be related to the up-regulation of Bax / Bcl-XL ratio to promote apoptosis.