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目的探讨褪黑素(Melatonin,MT)是否能减轻丙烯酰胺(ACR)导致的大鼠小脑的氧化损伤。方法 52只SD雄性大鼠按体重随机分为4个组,每组13只,分别为对照组、ACR组、MT组、MT+ACR联合组,均单笼饲养。对照组灌胃溶剂生理盐水,ACR组灌胃40 mg/kg ACR溶液,MT组腹腔注射5 mg/kg MT,MT+ACR联合组灌胃40 mg/kgACR溶液同时腹腔注射5 mg/kg MT,连续12 d。染毒结束后,断头处死大鼠,在冰盘上分离小脑,对小脑进行苏木素-伊红(HE)染色、氧化损伤检测和DNA损伤检测。结果 ACR组出现浦肯野细胞核固缩等异常改变,丙二醛(MDA)含量明显升高(P<0.05),T-SOD活性和GSH含量明显下降(P<0.05,P<0.01),彗星试验中尾长,尾部DNA百分含量及Olive尾距均显著增加,差异均有统计学意义(P<0.05或P<0.01)。MT+ACR组小脑结构正常,与ACR组相比,MT+ACR组MDA含量、彗星试验尾长、尾部DNA百分含量、Olive尾距均明显下降,T-SOD活性和GSH含量明显升高,差异均有统计学意义(P<0.05或P<0.01)。结论 ACR可以引起大鼠小脑结构改变、氧化损伤和DNA损伤,MT可以减轻ACR对小脑的结构损伤和氧化损伤。
Objective To investigate whether melatonin (MT) can reduce oxidative damage of rat cerebellum induced by acrylamide (ACR). Methods Fifty-two SD male rats were randomly divided into 4 groups according to body weight. Thirteen rats in each group were fed with control group, ACR group, MT group and MT + ACR group, respectively. The rats in control group were intragastrically infused with saline solution. The rats in ACR group were given 40 mg / kg ACR solution by intragastric administration. MT was intraperitoneally injected with 5 mg / kg MT. MT + ACR combined with intragastric administration of 40 mg / For 12 consecutive days. After the exposure, the rats were sacrificed and the cerebellum was isolated on ice tray. The cerebellum was stained with hematoxylin-eosin (HE), oxidative damage and DNA damage. Results The nuclear condensation and other abnormalities of Purkinje cell were observed in ACR group. The content of malondialdehyde (MDA) was significantly increased (P <0.05), the activity of T-SOD and the content of GSH were significantly decreased (P <0.05, P <0.01) Tail length, tail DNA content and Olive tail distance were significantly increased, the differences were statistically significant (P <0.05 or P <0.01). Compared with ACR group, the content of MDA in MT + ACR group, the length of tail in comet assay, the content of tail DNA and the tail of Olive in MT + ACR group were significantly decreased, the activity of T-SOD and GSH content were significantly increased, The differences were statistically significant (P <0.05 or P <0.01). Conclusion ACR can induce structural changes of cerebellum, oxidative damage and DNA damage in rats. MT can reduce the structural damage and oxidative damage of ACR to the cerebellum.