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利用本组1975年开始建立的小鼠可移植性前胃癌(FC)进行连续传代,对不同代数的自发转移进行比较观察.实验用615近交系小鼠99只,分为三组。第一组:小鼠39只,每只腋窝部皮下接种FC113代瘤细胞悬液0.1ml(细胞数为1.4×10~8)。第二、三组:小鼠各30只,瘤源分别为119代和124代,接种部位和剂量同第一组。动物分别于瘤株接种后第16天处死,将全肺及同、对侧腋窝淋巴结和部分动物局部肿瘤
Using this group of mouse transplantable precancerous gastric (FC) cells established in 1975 for continuous passage, spontaneous metastasis of different generations was observed. 99 inbred mice were divided into three groups. The first group consisted of 39 mice, each of which was inoculated subcutaneously with FC113 tumor cell suspension 0.1 ml (1.4×10-8 cells). In the second and third groups: 30 mice each. The tumor sources were 119 generations and 124 generations respectively. The inoculation site and dose were the same as the first group. The animals were sacrificed on the 16th day after the tumors were inoculated, and the whole lungs and the axillary lymph nodes in the same and contralateral sides and some local tumors in the animals were sacrificed.