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目的 分析非小细胞肺癌癌性胸水细胞中影响免疫状态的 7种细胞因子的表达 ,初步探讨肿瘤局部免疫微环境对抗肿瘤免疫应答的影响 ,揭示肿瘤逃逸机制。方法 应用原位杂交技术检测非小细胞肺癌癌性胸水和结核性胸膜炎炎性胸水细胞中IL 2、INF γ、IL 1 2、IL 1 8、IL 1 0、IL 4、TGF β1mRNA表达 ,比较其差异并进行半定量分析。结果 非小细胞肺癌癌性胸水细胞中IL 1 0、TGF β1、IL 4表达者显著高于IL 2、INF γ、IL 1 2、IL 1 8,且显著高于对照组。结核患者表达的各细胞因子水平均较低 ,且各因子的表达细胞数量相互间无显著性差异。结论 非小细胞肺癌患者癌性胸水细胞中Ⅱ型细胞因子表达者占主导地位 ,IL 1 0和TGF β1共同高表达 ,提示二者可能共同作用 ,参与形成非小细胞肺癌患者免疫抑制状态
Objective To analyze the expression of seven cytokines affecting immune status in cancerous pleural effusions of non-small cell lung cancer, and to explore the effect of local immune micro-environment on the immune response to tumors, and to reveal the mechanism of tumor escape. Methods In situ hybridization was used to detect the expression of IL-2, INF γ, IL12, IL18, IL10, IL4 and TGFβ1 mRNA in pleural effusion and tuberculous pleural pleural effusion in non-small cell lung cancer. Differences and semi-quantitative analysis. Results The expressions of IL 1 0, TGF β 1 and IL 4 in cancerous pleural effusions of non-small cell lung cancer were significantly higher than those of IL 2, INF γ, IL 12 and IL 8 and were significantly higher than those of the control group. The levels of cytokines expressed in TB patients were low, and there was no significant difference in the number of expression cells of each factor. Conclusions The expression of type II cytokines in cancerous pleural effusion cells is predominant in non-small cell lung cancer patients, IL 10 and TGF β1 are highly expressed together, suggesting that they may play a role in the formation of immunosuppressive status in patients with non-small cell lung cancer.