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目的:探讨超氧化物歧化酶(SOD)活性以及一氧化氮(NO)、丙二醛(MDA)和羟自由基(OH·)含量的变化与H3N2猪流感病毒诱导的小鼠肺损伤的关系。方法:选用6-8周龄、SPF级BALB/c小鼠80只,随机分为H3N2病毒感染急性肺损伤组和模拟感染对照组,每组各40只。在感染后的第3、5、7、10和14d,每组处死小鼠6只,做如下处理:其中2只小鼠取肺组织进行HE染色,观察肺组织的病理学变化;剩余4只小鼠处死,收集血液分离血清;然后进行支气管肺泡灌洗,收集支气管肺泡灌洗液(BALF)。测BALF和血清内SOD活性以及NO、MDA和OH·含量。结果:病毒感染小鼠肺脏组织学变化表现为以严重的肺泡间质水肿、炎性细胞渗出、出血为特征的弥漫性肺泡损伤;与模拟感染对照组相比,感染组BALF与血清内的NO、MDA和OH·的含量均明显增加,差异显著;感染组SOD活性与对照组相比显著下降。BALF内SOD、NO、MDA和OH·的变化幅度明显大于血清的变化。结论:感染小鼠血清和BALF内NO、MDA和OH·显著升高,表明在H3N2猪流感病毒诱导的小鼠肺损伤过程中上述自由基可能发挥重要作用。
Objective: To investigate the relationship between changes of superoxide dismutase (SOD) activity, nitric oxide (NO), malondialdehyde (MDA) and hydroxyl radical (OH ·) and lung injury induced by H3N2 swine influenza virus . Methods: Eighty BALB / c mice of 6-8 weeks old and SPF grade were randomly divided into three groups: n = 40 in each group and acute lung injury group infected with H3N2 virus. On the 3rd, 5th, 7th, 10th and 14th day after infection, 6 mice in each group were sacrificed and the following treatments were performed: 2 of them were taken lung tissue for HE staining to observe the pathological changes of lung tissue; the remaining 4 Mice were sacrificed and blood was collected to separate serum; bronchoalveolar lavage was then performed and bronchoalveolar lavage fluid (BALF) was collected. BALF and serum SOD activity and NO, MDA and OH · content were measured. Results: The histological changes in the lungs of mice infected with virus were diffuse alveolar lesions characterized by severe alveolar interstitial edema, inflammatory cell exudation and hemorrhage. Compared with the mock-infected control group, BALF and serum The content of NO, MDA and OH · significantly increased, the difference was significant. The SOD activity in infected group was significantly lower than that in control group. The changes of SOD, NO, MDA and OH · in BALF were significantly greater than those in serum. Conclusion: The levels of NO, MDA and OH in serum and BALF in infected mice were significantly increased, indicating that these free radicals may play an important role during the lung injury induced by H3N2 swine influenza virus.