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目的 :观察巴戟天对体外培养成骨细胞 (OB)增殖及其活性的影响 ,探讨巴戟天补肾壮骨作用的实质。方法 :取新生SD大鼠头盖骨提取成骨细胞 ,进行生长曲线分析 ,测定巴戟天对细胞增殖及分泌ALP、BGP、TGF β1的影响。结果 :巴戟天能显著刺激OB增殖 ;巴戟天与密钙息均可促进分化中的OB分泌ALP和骨钙素 ,促进OB表达TGF β1。结论 :巴戟天具有与密钙息相同的作用 ,即促进OB分泌ALP和骨钙素 ,促进OB表达TGF β1mRNA ,从而大量分泌Ⅰ型胶原 ,以利钙盐沉积
Objective : To observe the effect of Morinda officinalis on the proliferation and activity of osteoblasts (OB) in vitro, and to explore the essence of the effect of Basgu Tian Bushen Zhuang Gu. METHODS: Osteoblasts were extracted from the skull of neonatal SD rats and analyzed by growth curve. The effects of Morinda officinalis on proliferation and secretion of ALP, BGP and TGF β1 were determined. RESULTS: Morinda officinalis significantly stimulated OB proliferation. Morinda officinalis and denser calcium content both promoted the secretion of ALP and osteocalcin in differentiated OB and promoted the expression of TGF-beta1 by OB. Conclusion: Morinda officinalis has the same effect as dense calcium, ie, it promotes the secretion of ALP and osteocalcin by OB, promotes the expression of TGFβ1 mRNA by OB, and thus secretes large amount of type I collagen to facilitate the deposition of calcium salts.