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目的罗勒多糖具有抗肿瘤侵袭转移、化疗增效以及降血糖、降血脂和抗血栓等多种生物活性作用。本研究探讨罗勒多糖对转化生长因子-β(transforming growth factor-beta,TGF-β)诱导的人肺癌A549细胞侵袭运动能力的抑制作用,揭示罗勒多糖抑制上皮间质转化作用的可能机制。方法体外培养肺癌A549细胞,实验分为3组:(1)正常对照组,不加任何处理;(2)模型组,TGF-β5ng/mL;(3)罗勒多糖组,TGF-β5ng/mL+罗勒多糖200μg/mL。免疫荧光检测罗勒多糖对E-cadherin、Vimentin和α-SMA表达的影响,细胞侵袭实验和运动实验检测细胞对人工基底膜的降解和运动能力,细胞划痕实验检测细胞运动能力。结果与正常对照组比较,模型组细胞Vimentin和α-SMA表达增强,E-cadherin表达减弱。罗勒多糖处理后Vimentin和α-SMA表达减弱,E-cadherin表达增强。TGF-β可提高细胞降解人工基底膜的能力,单位视野下侵袭至下室的细胞数模型组为69.0±4.3,与正常对照组(42.0±4.30)和罗勒多糖组(37.0±4.41)比较,差异有统计学意义,均P<0.05。TGF-β增强了细胞的侵袭能力,罗勒多糖可以抑制TGF-β诱导的A549细胞侵袭能力。TGF-β使FN趋化的迁移细胞数目增多,单位视野下侵袭至下室的细胞数模型组为95.2±3.83,与正常对照组(69.2±5.11)和罗勒多糖组(68.2±6.18)比较,差异均有统计学意义,均P<0.05。划痕实验显示,TGF-β增强了细胞的迁移能力,罗勒多糖可以抑制TGF-β诱导的A549细胞迁移能力。结论罗勒多糖能够调节A549细胞上皮间质转化,这可能是其抑制A549细胞的侵袭转移能力的重要机制。
Objective Basil polysaccharides with anti-tumor invasion and metastasis, chemotherapy synergies and hypoglycemic, hypolipidemic and antithrombotic and other biological activity. This study was designed to investigate the inhibitory effect of basilain on invasion and migration of human lung cancer cell line A549 induced by transforming growth factor-beta (TGF-β), and to reveal the possible mechanism by which basilar polysaccharide can inhibit epithelial-mesenchymal transition. Methods The lung cancer A549 cells were cultured in vitro. The experiment was divided into three groups: (1) normal control group without any treatment; (2) model group, TGF-β5ng / mL; (3) basil group, TGF- Polysaccharide 200μg / mL. Immunofluorescence was used to detect the effect of basilar polysaccharide on the expression of E-cadherin, Vimentin and α-SMA. Cell invasion assay and exercise test were used to detect the degradation and motility of artificial basilar membrane. Cell scratch assay was used to detect cell motility. Results Compared with the normal control group, the expression of Vimentin and α-SMA increased and the expression of E-cadherin decreased in model group. The expression of Vimentin and α-SMA decreased after basil treatment, and the expression of E-cadherin was enhanced. TGF-β could improve the ability of cells to degrade artificial basement membrane. The number of cells invaded into the lower chamber in the unit field of vision was 69.0 ± 4.3. Compared with the normal control group (42.0 ± 4.30) and the basil group (37.0 ± 4.41) The difference was statistically significant, all P <0.05. TGF-β enhances the invasiveness of cells. Basil polysaccharide can inhibit TGF-β-induced invasiveness of A549 cells. TGF-β increased the number of migrating FN chemotactic cells, and the number of cells that infiltrated into the lower chamber per unit field of vision was 95.2 ± 3.83. Compared with the normal control group (69.2 ± 5.11) and the basal polysaccharide group (68.2 ± 6.18) The differences were statistically significant, all P <0.05. Scratch experiments showed that TGF-β enhanced cell migration ability, and basil could inhibit TGF-β-induced A549 cell migration. Conclusion Basil polysaccharide can regulate the epithelial-mesenchymal transition of A549 cells, which may be an important mechanism of its inhibition on the invasion and metastasis of A549 cells.