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目的 :对快肌和慢肌水溶性蛋白组进行比较 ,找出分别特异于快肌和慢肌的蛋白。方法 :应用蛋白组研究的核心技术———高分辨率二维电泳建立成年 (8月龄 )大鼠趾长伸肌 (代表快收缩纤维 )和比目鱼肌 (代表慢收缩纤维 )细胞内水溶性蛋白组表达的二维图谱。应用蛋白质氨基测序法和已知蛋白共电泳方法分别对经分离的蛋白进行鉴定。应用二维图谱差减法对快肌和慢肌的蛋白组进行比较。结果 :应用蛋白质氨基测序法和已知蛋白共电泳方法分别对 7个经分离的蛋白进行了鉴定。应用二维图谱差减法 ,发现了 3个分别特异于快肌和慢肌的蛋白。St1和St3仅在趾长伸肌被检测到 ,经蛋白质氨基测序法鉴定 ,St3为小钙白蛋白 ,而St2仅在比目鱼肌被检测到。结论 :快肌和慢肌细胞内水溶性蛋白组表达模式较为相似 ,但也分别存在其特异性蛋白 ,这些分别特异于快肌和慢肌的蛋白可能与其维持各自独特功能有关。小钙白蛋白和St1可作为鉴别快肌的标记物 ,而St2可作为鉴别慢肌的标记物。
OBJECTIVE: To compare fast-muscle and slow-muscle water-soluble proteins to find proteins that are specific for fast and slow muscles, respectively. METHODS: The intracellular water solubility of long and narrow extensor (representing fast-contracting) and soleus (representing slow-contractile) fibers in adult rats (8 months old) was established using high resolution two dimensional electrophoresis Two-dimensional map of proteome expression. The isolated protein was identified by amino acid sequencing and known protein co-electrophoresis. Compare the fast and slow muscle proteomes by two-dimensional map subtraction. Results: Seven isolated proteins were identified by amino acid sequencing and known protein co-electrophoresis. Applying the two-dimensional subtraction method, we found three proteins that are specific to fast and slow muscles, respectively. St1 and St3 were detected only in the extensor digitorum longus, identified by protein amino sequencing, St3 was small calpain, and St2 was detected only in the soleus muscle. CONCLUSION: The expression patterns of water-soluble proteins in fast and slow muscle cells are similar but their specific proteins exist separately. These proteins that are specific to fast and slow muscles may be related to their unique functions. Calcium and St1 can be used as markers to identify fast muscle, while St2 can be used as a marker to identify slow muscle.