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目的 建立T、B淋巴细胞联合免疫缺陷同源导入近交系 6 15 SCID小鼠。方法 利用经典遗传学的杂交 互交方法 ,将SCID小鼠携带的T、B淋巴细胞联合免疫缺陷基因 (scid基因 ) ,导入我国自己培育的 6 15近交系小鼠中。育种过程中采用白细胞计数分类及ELISA检测Ig含量方法 ,筛选每个M循环中的纯合子 ;再用蛋白质和同工酶电泳方法 ,分析纯合子位于 11个染色体上的 2 5个生化标记基因位点。结果 第 7个M循环中 ,所得纯合子与 6 15小鼠的 2 5个生化标记基因位点完全一致 ,表明M7循环的纯合子具有 6 15小鼠的全部标记基因。结论 M6、M7循环的纯合子小鼠表达scid基因 ,具有放射敏感性和T、B淋巴细胞联合缺陷的特征 ,且T细胞比例显著低于亲代SCID小鼠
Objective To establish T, B lymphocytes co-immunodeficiency homologous introduction of inbred 615 SCID mice. Methods T, B lymphocyte combined with immunodeficiency gene (scid gene) carried by SCID mice were introduced into 615 inbred mice of our own inoculation using classical genetics hybrid cross method. During the breeding process, the white blood cell count and ELISA method were used to detect Ig content. The homozygotes in each M cycle were screened out. The protein and isozyme electrophoresis method was used to analyze 25 biochemical marker loci on 11 chromosomes point. Results At the 7th M-cycle, the resulting homozygotes were completely identical to the 25 biochemical marker loci of 615 mice, indicating that the M7-loop homozygote had all the marker genes of 615 mice. Conclusion The homozygous mice in M6 and M7 cycles express the scid gene, which has the characteristics of radiosensitivity and T and B lymphocyte defects. The proportion of T cells is significantly lower than that of the parental SCID mice