论文部分内容阅读
目的建立高效液相色谱法同时测定保健食品中丹参酮ⅡA和丹酚酸B的含量。方法样品经75%甲醇-水(75∶25,V/V)超声提取,采用phenomenex Luna C_(18)色谱柱,以乙腈-0.1%(V/V)H_3PO_4溶液为流动相梯度洗脱,流速为1.0 ml/min,柱温为30℃,检测波长为286 nm、270 nm。结果丹参酮ⅡA和丹酚酸B分别在0.51μg/ml~25.4μg/ml、8.5μg/ml~318.8μg/ml时呈良好的线性关系(r=0.999 9、r=0.999 8)。低、中、高3种添加水平下加标回收率为94.2%~97.1%;相对标准偏差(RSD)为1.8%~2.6%。结论本方法简便、快捷、重复性好,可以用于保健食品中丹参酮ⅡA和丹酚酸B的含量测定。
Objective To establish a method for the simultaneous determination of tanshinone Ⅱ A and salvianolic acid B in health food by high performance liquid chromatography. Methods The samples were extracted with 75% methanol-water (75:25, V / V) and eluted with phenomenex Luna C 18 column with a mobile phase of acetonitrile -0.1% (V / V) H_3PO_4. 1.0 ml / min, column temperature was 30 ℃, detection wavelength was 286 nm, 270 nm. Results Tanshinone ⅡA and Salvianolic acid B showed a good linear relationship (r = 0.999 9, r = 0.999 8) at 0.51μg / ml ~ 25.4μg / ml and 8.5μg / ml ~ 318.8μg / ml respectively. The recoveries were 94.2% ~ 97.1% at low, medium and high levels, and the relative standard deviations (RSDs) were 1.8% ~ 2.6%. Conclusion The method is simple, rapid and reproducible. It can be used for the determination of tanshinone ⅡA and salvianolic acid B in health foods.