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目的采用高效液相-串联质谱方法测定血浆中法罗培南的药物浓度。方法色谱柱为ZORBAX Eclipse Plus(100mm×2.1mm,3.5μm),流动相:乙腈与5mmol/L醋酸铵缓冲液(含0.1%甲酸)体积比为35:65,流速为0.3ml/min,文拉法新为内标。结果法罗培南的血浆浓度在0.1～20μg/ml范围内线性良好,标准曲线方程为Y=0.2700c+0.00242(r=0.9955,n=8),定量下限为0.1μg/ml,日内精密度<5.4%,日间精密度<7.9%,回收率为99.0%～113%。结论该方法处理操作简单,结果准确,专属性强,灵敏度高,适用于法罗培南人体药动学研究及生物等效性研究。
Objective To determine the concentration of faropenem in plasma by high performance liquid chromatography-tandem mass spectrometry. Methods The column was ZORBAX Eclipse Plus (100mm × 2.1mm, 3.5μm). The mobile phase consisted of acetonitrile and 5mmol / L ammonium acetate buffer (containing 0.1% formic acid) in a volume ratio of 35:65 at a flow rate of 0.3ml / Rafah new internal standard. Results The plasma concentration of faropenem was linear in the range of 0.1 ~ 20μg / ml. The standard curve equation was Y = 0.2700c + 0.00242 (r = 0.9955, n = 8) and the limit of quantification was 0.1μg / %, Daytime precision <7.9%, recovery rate 99.0% ~ 113%. Conclusion The method is simple, accurate, specific and sensitive. It is suitable for the study of faropenem in human pharmacokinetics and bioequivalence studies.