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基因工程中，重组ＤＮＡ时，当外源ＤＮＡ很小并与载体相差很大时，其目的重组子的筛选鉴定工作不仅量大，而且常规鉴定方法无法采用。本文针对此类重组子的鉴定方法作了探讨，提出了三级四步筛选鉴定法，即：质粒筛选、重组鉴定、目的重组鉴定和双酶切鉴定。该法不仅有效地解决了国内普通实验条件下含小插入片段重组子的鉴定问题，大大缩小鉴定范围，而且快速省耗的鉴定方法也为ｐＧＥＸ载体系统的更广泛应用提供了新的启示。
Genetic engineering, recombinant DNA, when the exogenous DNA is very small and very different from the vector, the purpose of screening of recombinant recombination is not only a large amount, and conventional identification methods can not be used. In this paper, the methods of identification of such recombinants were discussed, and three-step four-step screening and identification methods were proposed, namely: plasmid screening, recombinant identification, target recombinant identification and double enzyme digestion. The method not only effectively solves the problem of identification of recombinants with small insertions under the general experimental conditions in China, but also greatly reduces the scope of identification. Moreover, the rapid and efficient identification method also provides new enlightenment for the wider application of pGEX vector system.