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建立抗稻瘟病基因的分子标记对培育抗稻瘟病品种有重要意义。研究利用71个广西地方菌株检测地谷对稻瘟病菌的抗性,并利用地谷中Pi-d2基因与广恢998等位基因在基因组序列上一个碱基的差异,设计出以抗病基因本身序列为引物的基因标签M-Pid2,通过分析地谷与广恢998的F2群体的基因型与对稻瘟病抗感分离的吻合度来验证该标签的准确性,用M-Pid2扩增62份水稻种质验证该标签的特异性。结果表明,地谷能抗71个地方稻瘟病菌株中的59个菌株,抗性频率达83.1%。用M-Pid2扩增地谷与广恢998的F2群体的基因型与其对稻瘟病的抗感分离完全吻合;用M-Pid2扩增62份水稻种质,仅在地谷中扩增出629bp特异条带。从而确认抗稻瘟病基因Pi-d2为地谷的主效抗稻瘟病基因,且在广西有育种利用价值;标记M-Pid2能准确用于抗稻瘟病基因Pi-d2的辅助选择。
It is of great significance to establish the molecular marker of blast resistance genes to cultivate the blast resistant varieties. In this study, 71 Guangxi local isolates were used to test the resistance of Upland rice to Magnaporthe grisea. Based on the difference in base sequence of Pi-d2 gene and U-hu 998 alleles in Diya, The sequence was the gene tag M-Pid2 of the primer, and the accuracy of the tag was verified by analyzing the genotypes of the F2 population of DiGu Guang and Guang Hui 998 against the anti-flu isolation of rice blast, and 62 copies were amplified by M-Pid2 Rice germplasm verifies the specificity of the tag. The results showed that Vigor can resist 59 isolates from 71 rice blast isolates, with a frequency of 83.1%. Genotypes of F2 population amplified with M-Pid2 were completely consistent with their resistance to rice blast; 62 rice germplasms were amplified with M-Pid2 and 629 bp specific Bands. Thus, it was confirmed that Pi-d2 was the main blast-resistance gene in Gutiao and it had the value of breeding in Guangxi. The marker M-Pid2 could be accurately used for the auxiliary selection of the blast resistance gene Pi-d2.