论文部分内容阅读
目的： 探讨ｐ１６ 基因是否在鼻咽癌活检组织中存在高频的失活， 以确定该基因在鼻咽癌发病过程中所起的作用。方法：首先利用免疫组化的方法检测了１４ 例鼻咽癌活检组织中ｐ１６ 基因的表达状况。并用比较多重 Ｐ Ｃ Ｒ Ｓｏｕｔｈｅｒｎ 杂交的方法检测了这１４ 例鼻咽癌活检组织中ｐ１６ 基因外显子１α和外显子２ 可能的缺失， 以了解导致ｐ１６ 基因表达下调的机制。结果：在１４ 例鼻咽癌活检组织中有１２ 例（ ～８５ ％ ） 缺乏ｐ１６ 蛋白的表达，但其中只有２ 例鼻咽癌活检组织存在外显子２ 的缺失。结论：以上的数据显示ｐ１６ 基因在鼻咽癌中存在高频率的失活，提示该基因在鼻咽癌发病过程中具有一定的作用， 同时， 上述结果也提示ｐ１６ 基因的缺失并不是鼻咽癌中造成ｐ１６基因表达下调的主要原因。
Objective: To investigate whether p16 gene has high-frequency inactivation in nasopharyngeal carcinoma biopsies to determine its role in the pathogenesis of nasopharyngeal carcinoma. Methods: Firstly, the expression of p16 gene in 14 cases of nasopharyngeal carcinoma biopsies was detected by immunohistochemistry. The possible deletions of exon 1 and exon 2 of p16 gene in the biopsy tissues of nasopharyngeal carcinoma were detected by multiple PCR-Southern hybridization to find out the mechanism of down-regulation of p16 gene expression. RESULTS: Twelve (~ 85%) of the 14 NPC biopsies lacked the expression of p16 protein, but only 2 of them had a deletion of exon 2 in the biopsy tissue of NPC. Conclusion: The above data show that the high frequency of p16 gene inactivation of nasopharyngeal carcinoma, suggesting that the gene plays a role in the pathogenesis of nasopharyngeal carcinoma, at the same time, the above results also suggest that the deletion of p16 gene is not nasopharyngeal carcinoma Caused in the main reason for the down-regulation of p16 gene.