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目的:探讨骨髓间充质干细胞(BSMCs)向C6胶质瘤定向迁移的能力以及DAPI用于BMSCs向胶质瘤体内迁移示踪的价值。方法:直接贴壁法分离培养纯化BMSCs。利用Transwell小室建立体外迁移模型检测BMSCs向C6胶质瘤细胞定向迁移的能力。立体定向法建立大鼠C6胶质瘤模型,利用DAPI体外标记培养、纯化的BMSCs,经荷瘤侧颈内动脉灌注,观察BMSCs向C6胶质瘤组织的定向迁移能力,并评价这一过程中DAPI用于BMSCs标记的价值。结果:通过直接贴壁法分离、培养、传代,获得了纯化的BMSCs。体外迁移实验证实,BMSCs具有向C6胶质瘤细胞定向迁移的能力,迁移细胞数量为400倍视野下(32.1±10.5)/HP。DAPI标记后BMSCs细胞核呈蓝色荧光,阳性率达100%。经荷瘤大鼠颈内动脉灌注后BMSCs可以存活,并表现出向脑胶质瘤趋化迁移的特性,分布区域主要位于肿瘤内部血管周边。结论:DAPI可以用于BMSCs的体内示踪,BMSCs具有通过血肿瘤屏障向C6胶质瘤定向迁移的能力,经颈内动脉灌注是其有效的移植途径。
Objective: To investigate the ability of bone marrow mesenchymal stem cells (BSMCs) to migrate to C6 glioma and the value of DAPI in the migration of BMSCs into glioma. Methods: BMSCs were isolated and purified directly by adherent method. Transwell chamber was used to establish an in vitro migration model to detect the migration of BMSCs to C6 glioma cells. The rat C6 glioma model was established by stereotactic method. The BMSCs cultured in vitro and purified by DAPI were used to observe the directional migration of BMSCs into C6 glioma tissue. The value of DAPI for labeling BMSCs. Results: The purified BMSCs were isolated, cultured and passaged by direct adherent method. In vitro migration experiments confirmed that BMSCs have the ability of directional migration of C6 glioma cells, the number of migrating cells is 400 times the field of vision (32.1 ± 10.5) / HP. After DAPI labeling, BMSCs nuclei showed blue fluorescence with a positive rate of 100%. BMSCs survived after carotid artery perfusion in tumor-bearing rats and showed the chemotactic migration to glioma. The distribution area mainly located in the perivascular of the tumor. CONCLUSION: DAPI can be used for in vivo tracing of BMSCs. BMSCs have the ability to migrate to the C6 glioma through the blood-tumor barrier. Perfusion through the internal carotid artery is an effective transplantation route.