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目的探讨盐霉素对结肠癌干细胞(cCSC)的作用。方法通过无血清培养基(SFM)成球法筛选cCSC。检测盐霉素对cCSC的靶向性,并进一步研究盐霉素对cCSC的增殖能力、耐药性、克隆球形成能力、干细胞相关蛋白表达水平的作用。结果通过SFM成球法成功筛选出了cCSC,建立了理想的cCSC模型,cCSC对盐霉素的敏感性是HCT116+/+细胞的3倍。经盐霉素处理后,cCSC倍增时间从(1.21±0.15)d延长到了(1.54±0.04)d;对氟尿嘧啶(5-FU)的IC50从(40.21±3.02)μmol/L降到了(20.28±2.15)μmol/L,对奥沙利铂的IC50从(14.23±1.08)μmol/L降到(7.64±0.53)μmol/L;克隆球形成率由从(0.42±0.01)%下降到了(0.08±0.01)%;干细胞相关蛋白ABCG2、SOX2和OCT4表达水平明显降低。结论盐霉素对cCSC具有明显的靶向性,明显抑制了cCSC的增殖能力、耐药性、克隆球形成能力及干细胞相关蛋白的表达,为cCSC治疗提供了新的理论基础。
Objective To investigate the effect of salinomycin on colon cancer stem cells (cSCs). Methods Screened cCSCs by serum-free medium (SFM). To detect the targeting of salinomycin to cCSC, and to further study the effect of salinomycin on cSCC proliferation, drug resistance, the ability of cloning sphere formation and stem cell-related protein expression. Results The cCSC was successfully screened by SFM spheroids and the ideal model of cCSC was established. The sensitivity of cCSC to salinomycin was three times that of HCT116 + / + cells. After salinomycin treatment, the doubling time of cCSC increased from (1.21 ± 0.15) d to (1.54 ± 0.04) d, and the IC50 of 5-fluorouracil decreased from (40.21 ± 3.02) μmol / L to (20.28 ± 2.15 ) decreased the IC50 of oxaliplatin from (14.23 ± 1.08) μmol / L to (7.64 ± 0.53) μmol / L; the rate of formation of the spheres decreased from (0.42 ± 0.01)% to )%. The expressions of stem cell related proteins ABCG2, SOX2 and OCT4 were significantly decreased. Conclusion Salinomycin has obvious targeting on cCSC and significantly inhibits the proliferation, drug resistance, the ability of cloning sphere formation and the expression of stem cell related proteins in cCSC, which provides a new theoretical basis for the treatment of cCSC.