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为观察大鼠大脑运动皮质向脑干运动前神经元所在区域的谷氨酸能投射。用逆行追踪和免疫荧光组织化学染色相结合的双标技术，将四甲基罗达明（ＴＭＲ）分别注入臂旁外侧核、三叉上核或延髓网状结构外侧部后，ＴＭＲ逆标神经元分别见于额叶２区、额叶１区或额叶１区和２区的Ｖ层；磷酸激活的谷氨酰胺酶（ＰＡＧ）样阳性神经元见于大脑运动皮质Ⅱ～Ⅵ层的锥体细胞；ＴＭＲ逆标神经元几乎均呈ＰＡＧ样阳性。结果提示，大脑运动皮质发出的皮质脑干束主要终止于脑干运动前神经元所在的区域，运动皮质向不同的脑干运动前神经元所在区域发出投射的起源也有差异，谷氨酸是此通路的主要神经递质。
In order to observe the glutamate projection of rat brain cortex before the brainstem motor neurons. TMR retrogradely labeled neurons were respectively labeled with tetramethylrhodamine (TMR) into lateral parabrachial nucleus, supractorial nucleus, or medulla oblongata by double labeling technique with retrograde tracing and immunofluorescence histochemistry staining Frontal lobe 2, frontal lobe 1 or frontal lobe 1 and 2. The phosphorylated glutamase (PAG) -like neurons were found in the pyramidal cells of layers II-VI of the motor cortex. The TMR inverse Nearly all of the standard neurons showed PAG-like positive. The results suggest that cortical brainstem cortex emitted from cerebral motor cortex mainly terminates in the area of neurons before brainstem motility. There are also differences in the origin of motor cortex to different areas of brain stem pre-motor neurons. Glutamate is The main neurotransmitter of the pathway.