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系统考察了功能单体对非共价分子烙印手性固定相手性分离能力的影响,发现在非共价分子烙印手性固定相的制备中,功能单体与烙印分子之间存在着匹配性.丙烯酰胺可以与氨基酸衍生物的酰胺基团形成较强的氢键作用,碱性功能单体2-乙烯基吡啶则与其羧基形成较强的离子作用,两者的协同作用使复合功能单体丙烯酰胺+2-乙烯基吡啶对于氨基酸衍生物具有良好的烙印效果.竞争溶剂乙酸对样品与分子烙印固定相间的非共价作用力有较大的影响,增加流动相中的竞争溶剂乙酸的含量,将减弱分子烙印手性固定相与样品的酰胺键和羧基的氢键及离子作用,导致对样品的容量因子、手性选择性α及分离度f/g的减小.
The influence of functional monomers on the chiral separation ability of non-covalent molecular imprinted chiral stationary phases was systematically investigated. It was found that there was a match between functional monomers and imprinted molecules in the preparation of non-covalent molecular imprinted chiral stationary phases. Acrylamide can form strong hydrogen bond with the amide group of amino acid derivatives. The basic functional monomer 2-vinyl pyridine forms a strong ionic interaction with its carboxyl group, and the synergistic effect of the two can make the composite functional monomer propylene Amide + 2-vinylpyridine has a good imprinting effect on amino acid derivatives.The competitive solvent acetic acid has a significant influence on non-covalent forces between the sample and the molecularly imprinted stationary phase, increases the content of acetic acid, a competitive solvent in the mobile phase, Will weaken the hydrogen bonding and ionic interaction between the molecularly imprinted chiral stationary phase and the amide and carboxyl groups of the sample, resulting in a decrease of the capacity factor, the chiral selectivity α and the separation f / g of the sample.